J Cancer 2018; 9(20):3647-3650. doi:10.7150/jca.27052

Research Paper

Variants in the 3'-untranslated region of CUL3 is associated with risk of esophageal squamous cell carcinoma

Jin-Long Hu1✉, Xin-Long Hu2, Chuang-Xin Lu1, Xue-Jiao Chen1, Lei Fu1, Qian Han3, Shun-Dong Cang1✉

1. Department of Oncology, People's Hospital of Zhengzhou University and Henan Provincial People's Hospital, Zhengzhou 450000, P. R. China
2. Department of Medical Imaging Technology, Henan University of Chinese Medicine, Zhengzhou 450000, P. R. China
3. Department of Radiotherapy, People's Hospital of Zhengzhou University and Henan Provincial People's Hospital, Zhengzhou 450000, P. R. China

Abstract

Background: Esophageal squamous cell carcinoma (ESCC) is one of the most lethal cancers in China. Recently, a study identified that cullin 3 (CUL3) was significantly mutated and deleted in ESCC. We then hypothesis that germline variants in CUL3 may also associated with the susceptibility of ESCC. Variants in the gene 3'-untranslated region (3'-UTR) may associate with gene expression by altering miRNAs binding.

Material and Methods: We systematically searched for variants in the 3'-UTR of CUL3 using the Ensembl database. Taqman SNP Genotyping Assay was performed in 638 ESCC cases and 546 controls to examine the association between the rs2396092 and the risk of ESCC. The eQTL analysis for CUL3 were conducted by using the GTEx database.

Results: We identified that the rs2396092 was significantly associated with the susceptibility of ESCC. Compared with the TT genotype carriers, the CT genotype and CC genotype carriers were correlated with risk of ESCC with odds ratio being 1.33 (95% CI: 1.04-1.70, P=0.0222) and 1.63 (95% CI: 1.07-2.50, P=0.0241), respectively. Different genotypes of rs2396092 was also shown to be correlated with altered CUL3 expression.

Conclusion: The results emphasize the importance of CUL3 in the development of ESCC and may contribute to the personalized prevention of this cancer in the future.

Keywords: Esophageal squamous cell carcinoma, SNP, Susceptibility, CUL3, 3'UTR

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How to cite this article:
Hu JL, Hu XL, Lu CX, Chen XJ, Fu L, Han Q, Cang SD. Variants in the 3'-untranslated region of CUL3 is associated with risk of esophageal squamous cell carcinoma. J Cancer 2018; 9(20):3647-3650. doi:10.7150/jca.27052. Available from http://www.jcancer.org/v09p3647.htm