J Cancer 2021; 12(9):2723-2734. doi:10.7150/jca.55626 This issue Cite

Research Paper

LINC01296/miR-141-3p/ZEB1-ZEB2 axis promotes tumor metastasis via enhancing epithelial-mesenchymal transition process

Zhenqiang Sun1,4✉, Bo Shao1,4, Zaoqu Liu3, Qin Dang1,4, Yaxin Guo5,6, Chen Chen2,4, Yuying Guo1, Zhuang Chen1, Jinbo Liu1, Shengyun Hu1, Weitang Yuan1✉, Quanbo Zhou1✉

1. Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
2. School of Life Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China.
3. Department of Interventional Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.
4. Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450052, Henan, China.
5. Department of Basic Medical, Academy of Medical Sciences of Zhengzhou University, Zhengzhou 450052, Henan, China.
6. Henan Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450052, Henan, China.

Citation:
Sun Z, Shao B, Liu Z, Dang Q, Guo Y, Chen C, Guo Y, Chen Z, Liu J, Hu S, Yuan W, Zhou Q. LINC01296/miR-141-3p/ZEB1-ZEB2 axis promotes tumor metastasis via enhancing epithelial-mesenchymal transition process. J Cancer 2021; 12(9):2723-2734. doi:10.7150/jca.55626. https://www.jcancer.org/v12p2723.htm
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Abstract

Graphic abstract

Purpose: Tumor metastasis seriously affects the survival of patients. In recent years, some studies confirmed that long non-coding RNA (lncRNA) played an essential role in tumor progression. A few studies reported that LINC01296 acted as an oncogenic regulator of cancer. However, its in-depth specific biological mechanism in tumor metastasis is still unknown.

Methods: Real-time quantitative PCR (qPCR) was performed to detect the expression of LINC01296 and miR-141-3p in NSCLC, CRC tissues and cell lines, and the dual luciferase report was used to evaluate the relationship between LINC01296, miR-141-3p and ZEB1/ZEB2 relationship. Western blot experiments are used to detect changes in protein levels. Transwell and wound healing measures migration and invasion capabilities.

Results: In this study, we used non-small cell lung cancer (NSCLC) and colorectal cancer (CRC) as the research objects, LINC01296 was found to be highly expressed in NSCLC and CRC tissues and positively related to poor prognosis. We also demonstrated LINC01296 regulated NSCLC and CRC invasion and metastasis by modulating epithelial-mesenchymal transition (EMT) by up-regulating ZEB1 and ZEB2. Consequently, LINC01296 acted as a sponge of miR-141-3p, which negatively regulates EMT process.

Conclusions: The report revealed a new mechanism by which LINC01296 regulates the EMT process through miR-141-3p/ZEB1-ZEB2 axis and affects cancer metastasis.

Keywords: tumor metastasis, long noncoding RNA, non-small cell lung cancer, colorectal cancer, epithelial-mesenchymal transition


Citation styles

APA
Sun, Z., Shao, B., Liu, Z., Dang, Q., Guo, Y., Chen, C., Guo, Y., Chen, Z., Liu, J., Hu, S., Yuan, W., Zhou, Q. (2021). LINC01296/miR-141-3p/ZEB1-ZEB2 axis promotes tumor metastasis via enhancing epithelial-mesenchymal transition process. Journal of Cancer, 12(9), 2723-2734. https://doi.org/10.7150/jca.55626.

ACS
Sun, Z.; Shao, B.; Liu, Z.; Dang, Q.; Guo, Y.; Chen, C.; Guo, Y.; Chen, Z.; Liu, J.; Hu, S.; Yuan, W.; Zhou, Q. LINC01296/miR-141-3p/ZEB1-ZEB2 axis promotes tumor metastasis via enhancing epithelial-mesenchymal transition process. J. Cancer 2021, 12 (9), 2723-2734. DOI: 10.7150/jca.55626.

NLM
Sun Z, Shao B, Liu Z, Dang Q, Guo Y, Chen C, Guo Y, Chen Z, Liu J, Hu S, Yuan W, Zhou Q. LINC01296/miR-141-3p/ZEB1-ZEB2 axis promotes tumor metastasis via enhancing epithelial-mesenchymal transition process. J Cancer 2021; 12(9):2723-2734. doi:10.7150/jca.55626. https://www.jcancer.org/v12p2723.htm

CSE
Sun Z, Shao B, Liu Z, Dang Q, Guo Y, Chen C, Guo Y, Chen Z, Liu J, Hu S, Yuan W, Zhou Q. 2021. LINC01296/miR-141-3p/ZEB1-ZEB2 axis promotes tumor metastasis via enhancing epithelial-mesenchymal transition process. J Cancer. 12(9):2723-2734.

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