J Cancer 2021; 12(9):2756-2767. doi:10.7150/jca.48419 This issue Cite

Research Paper

SAA1 knockdown promotes the apoptosis of glioblastoma cells via downregulation of AKT signaling

Huikai Zhang1,2, Yang Xu1,2, Gang Deng1,2, Fanen Yuan1,2, Yinqiu Tan1,2, Lun Gao1,2, Qian Sun1,2, Yangzhi Qi1,2, Kun Yang1,2, Rongxin Geng1,2, Hongxiang Jiang1,2, Baohui Liu1,2✉, Qianxue Chen1,2✉

1. Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, China.
2. Central Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.

Citation:
Zhang H, Xu Y, Deng G, Yuan F, Tan Y, Gao L, Sun Q, Qi Y, Yang K, Geng R, Jiang H, Liu B, Chen Q. SAA1 knockdown promotes the apoptosis of glioblastoma cells via downregulation of AKT signaling. J Cancer 2021; 12(9):2756-2767. doi:10.7150/jca.48419. https://www.jcancer.org/v12p2756.htm
Other styles

File import instruction

Abstract

Graphic abstract

Serum amyloid A1 (SAA1) is an inflammatory associated high-density lipoprotein. And It is also considered as a predictor and prognostic marker of cancer risk. However, its role and mechanisms in glioblastoma (GBM) still unclear. In this study, we validate that SAA1 is up-regulated in GBM, and its high expression predicts poor prognosis. SAA1 knockdown promotes the apoptosis of GBM cell. Mechanistically, SAA1 knockdown can inhibit serine/threonine protein kinase B (AKT) phosphorylation, thereby regulating the expression of apoptosis-related proteins such as Bcl2 and Bax, leading to GBM cell death. Moreover, Gliomas with low SAA1 expression have increased sensitivity to Temozolomide (TMZ). Low SAA1 expression segregated glioma patients who were treated with Temozolomide (TMZ) or with high MGMT promoter methylation into survival groups in TCGA and CGGA dataset. Our study strongly suggested that SAA1 was a regulator of cells apoptosis and acted not only as a prognostic marker but also a novel biomarker of sensitivity of glioma to TMZ.

Keywords: Serum amyloid A1, Glioblastoma, Apoptosis, AKT, Temozolomide.


Citation styles

APA
Zhang, H., Xu, Y., Deng, G., Yuan, F., Tan, Y., Gao, L., Sun, Q., Qi, Y., Yang, K., Geng, R., Jiang, H., Liu, B., Chen, Q. (2021). SAA1 knockdown promotes the apoptosis of glioblastoma cells via downregulation of AKT signaling. Journal of Cancer, 12(9), 2756-2767. https://doi.org/10.7150/jca.48419.

ACS
Zhang, H.; Xu, Y.; Deng, G.; Yuan, F.; Tan, Y.; Gao, L.; Sun, Q.; Qi, Y.; Yang, K.; Geng, R.; Jiang, H.; Liu, B.; Chen, Q. SAA1 knockdown promotes the apoptosis of glioblastoma cells via downregulation of AKT signaling. J. Cancer 2021, 12 (9), 2756-2767. DOI: 10.7150/jca.48419.

NLM
Zhang H, Xu Y, Deng G, Yuan F, Tan Y, Gao L, Sun Q, Qi Y, Yang K, Geng R, Jiang H, Liu B, Chen Q. SAA1 knockdown promotes the apoptosis of glioblastoma cells via downregulation of AKT signaling. J Cancer 2021; 12(9):2756-2767. doi:10.7150/jca.48419. https://www.jcancer.org/v12p2756.htm

CSE
Zhang H, Xu Y, Deng G, Yuan F, Tan Y, Gao L, Sun Q, Qi Y, Yang K, Geng R, Jiang H, Liu B, Chen Q. 2021. SAA1 knockdown promotes the apoptosis of glioblastoma cells via downregulation of AKT signaling. J Cancer. 12(9):2756-2767.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Popup Image