J Cancer 2011; 2:262-270. doi:10.7150/jca.2.262

Research Paper

Cellular and Phenotypic Characterization of Canine Osteosarcoma Cell Lines

Marie E. Legare, Jamie Bush, Amanda K. Ashley, Taka Kato, William H. Hanneman

The Center for Environmental Medicine, Department of Environmental and Radiological Health Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA

Abstract

Canine and human osteosarcoma (OSA) have many similarities, with the majority of reported cases occurring in the appendicular skeleton, gender predominance noted, high rate of metastasis at the time of presentation, and a lack of known etiology for this devastating disease. Due to poor understanding of the molecular mechanisms underlying OSA, we have characterized seven different OSA canine cell lines: Abrams, D17, Grey, Hughes, Ingles, Jarques, and Marisco and compared them to U2, a human OSA cell line, for the following parameters: morphology, growth, contact inhibition, migrational tendencies, alkaline phosphatase staining, heterologous tumor growth, double-strand DNA breaks, and oxidative damage. All results demonstrated the positive characteristics of the Abrams cell line for use in future studies of OSA. Of particular interest, the robust growth of a subcutaneous tumor and rapid pulmonary metastasis of the Abrams cell line in an immunocompromised mouse shows incredible potential for the future use of Abrams as a canine OSA model. Further investigations utilizing a canine cell model of OSA, such as Abrams, will be invaluable to understanding the molecular events underlying OSA, pharmaceutical inhibition of metastasis, and eventual prevention of this devastating disease.

Keywords: Abrams, Canine, Osteosarcoma, U2

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How to cite this article:
Legare ME, Bush J, Ashley AK, Kato T, Hanneman WH. Cellular and Phenotypic Characterization of Canine Osteosarcoma Cell Lines. J Cancer 2011; 2:262-270. doi:10.7150/jca.2.262. Available from http://www.jcancer.org/v02p0262.htm