ISSN: 1837-9664Journal of Cancer
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J Cancer 2016; 7(4):462-466. doi:10.7150/jca.13566 This issue Cite
Research Paper
Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent
1. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia;
2. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia;
3. Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.
Citation:
Baradaran A, Yusoff K, Shafee N, Rahim RA. Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent. J Cancer 2016; 7(4):462-466. doi:10.7150/jca.13566. https://www.jcancer.org/v07p0462.htm
Other stylesAbstract
The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) with its immunotherapeutic activities and sialic acid binding abilities is a promising cancer adjuvant. The HN was surfaced displayed on Lactococcus lactis and its cancer targeting ability was investigated via attachment to the MDA-MB231 breast cancers. To surface display the HN protein on the bacterial cell wall, HN was fused to N-acetylmuraminidase (AcmA) anchoring motif of L. lactis and expressed in Chinese hamster ovary cells. The expressed recombinant fusion proteins were purified and mixed with a culture of L. lactis and Lactobacillus plantarum. Immunofluorescence assay showed the binding of the recombinant HN-AcmA protein on the surface of the bacterial cells. The bacterial cells carrying the HN-AcmA protein interacted with the MDA-MB231 breast cancer cells. Direct and fluorescent microscopy confirmed that L. lactis and Lb. plantarum surface displaying the recombinant HN were attached to the breast cancer MDA-MB231 cells, providing evidence for the potential ability of HN in targeting to cancer cells.
Keywords: Hemagglutinin-neuraminidase, Cancer targeting, Lactococcus lactis, Surface display.
Citation styles
APA
Baradaran, A., Yusoff, K., Shafee, N., Rahim, R.A. (2016). Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent. Journal of Cancer, 7(4), 462-466. https://doi.org/10.7150/jca.13566.
ACS
Baradaran, A.; Yusoff, K.; Shafee, N.; Rahim, R.A. Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent. J. Cancer 2016, 7 (4), 462-466. DOI: 10.7150/jca.13566.
NLM
Baradaran A, Yusoff K, Shafee N, Rahim RA. Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent. J Cancer 2016; 7(4):462-466. doi:10.7150/jca.13566. https://www.jcancer.org/v07p0462.htm
CSE
Baradaran A, Yusoff K, Shafee N, Rahim RA. 2016. Newcastle Disease Virus Hemagglutinin Neuraminidase as a Potential Cancer Targeting Agent. J Cancer. 7(4):462-466.
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