High Stoichiometry Phosphorylation of Talin at T144/T150 or S446 Produces Contrasting Effects on Calpain-mediated Talin Cleavage and Cell Migration

Li, Youjun; Luo, Xiaoyong; Sun, Yang; Cui, Zhenyi; Liu, Yizhou; Liu, Rushi; Guo, Xiangrong

doi:10.7150/jca.14192

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J Cancer 2016; 7(12):1645-1652. doi:10.7150/jca.14192 This issue Cite

Research Paper

High Stoichiometry Phosphorylation of Talin at T144/T150 or S446 Produces Contrasting Effects on Calpain-mediated Talin Cleavage and Cell Migration

Youjun Li¹, Xiaoyong Luo¹, Yang Sun¹, Zhenyi Cui¹, Yizhou Liu¹, Rushi Liu^2✉, Xiangrong Guo^1✉

1. Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hunan Normal University, Changsha 410081, China
2. Laboratory of Medical Molecular and Immunological Diagnostics, College of Medicine, Hunan Normal University, Changsha 410013, China

Citation:

Li Y, Luo X, Sun Y, Cui Z, Liu Y, Liu R, Guo X. High Stoichiometry Phosphorylation of Talin at T144/T150 or S446 Produces Contrasting Effects on Calpain-mediated Talin Cleavage and Cell Migration. J Cancer 2016; 7(12):1645-1652. doi:10.7150/jca.14192. https://www.jcancer.org/v07p1645.htm

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Abstract

Focal adhesions are large multi-protein complexes that serve as the linkage between extracellular matrix (ECM) and actin cytoskeleton and control the network of signaling cascades underlying cell migration. Talin plays a key role in focal adhesion turnover, and calpain-mediated proteolysis of talin is central to focal adhesion disassembly, but its regulation is not well elucidated. Here we demonstrate that talin phosphorylation at three high stoichiometry sites on its head domain, T144 and T150, or S446, have contrasting effects on calpain-mediated cleavage of talin and cell migration by using site-directed mutagenesis to inhibit phosphorylation. Expression of talin^T144A+T150A stimulated calpain-mediated cleavage of talin and accelerated focal adhesion disassembly, whereas expression of talin^S446A fully inhibited talin cleavage by calpain, preventing focal adhesion disassembly. A large decrease in phospho-threonine or phospho-serine levels was seen with talin^T144A+T150A or talin^S446A respectively, while more active ERK was present in talin^T144A+T150A than in talin^S446A. Cell adhesion and transwell assays using uniformly expressing cells showed that expression of talin^T144A+T150A or talin^S446A have opposing effects on cell adhesion and migration. These findings define and highlight the integral role of site-specific high stoichiometry phosphorylation of talin in regulating calpain-mediated cleavage of talin and focal adhesion disassembly, thus controlling adhesion stability, cell adhesion and ultimately, cell migration.

Keywords: Talin, High stoichiometry phosphorylation, Calpain-mediated cleavage, Focal adhesion disassembly

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APA

Li, Y., Luo, X., Sun, Y., Cui, Z., Liu, Y., Liu, R., Guo, X. (2016). High Stoichiometry Phosphorylation of Talin at T144/T150 or S446 Produces Contrasting Effects on Calpain-mediated Talin Cleavage and Cell Migration. Journal of Cancer, 7(12), 1645-1652. https://doi.org/10.7150/jca.14192.

ACS

Li, Y.; Luo, X.; Sun, Y.; Cui, Z.; Liu, Y.; Liu, R.; Guo, X. High Stoichiometry Phosphorylation of Talin at T144/T150 or S446 Produces Contrasting Effects on Calpain-mediated Talin Cleavage and Cell Migration. J. Cancer 2016, 7 (12), 1645-1652. DOI: 10.7150/jca.14192.

NLM

CSE

Li Y, Luo X, Sun Y, Cui Z, Liu Y, Liu R, Guo X. 2016. High Stoichiometry Phosphorylation of Talin at T144/T150 or S446 Produces Contrasting Effects on Calpain-mediated Talin Cleavage and Cell Migration. J Cancer. 7(12):1645-1652.

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