J Cancer 2017; 8(3):363-370. doi:10.7150/jca.16730

Research Paper

High Infiltration of Polarized CD163+ Tumor-Associated Macrophages Correlates with Aberrant Expressions of CSCs Markers, and Predicts Prognosis in Patients with Recurrent Gastric Cancer

Wei-jie Zhang1, 2*, Zhi-hua Zhou3*, Ming Guo4, Liu-qing Yang5, Yuan-yuan Xu5, Tao-hong Pang5, Shan-ting Gao2, Xin-yun Xu6, Qi Sun6, Min Feng1, 2, Hao Wang1, 2, Chun-lei Lu7, Guo-zhong Wu7✉, Wen-xian Guan1,2✉, Gui-fang Xu5✉

1. Department of General Surgery, Drum Tower Clinical College of Nanjing Medical University, Nanjing, Jiangsu Province, China;
2. Department of General Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China;
3. Department of Pathology, 101th Hospital of PLA, Wuxi, Jiangsu Providence, China;
4. Department of Gastroenterology, People' s Hospital of Anji, Huzhou, Zhejiang Province, China;
5. Department of Gastroenterology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China;
6. Department of Pathology, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China;
7. Department of General Surgery, 101th Hospital of PLA, Wuxi, Jiangsu Province, China.
* Wei-jie Zhang and Zhi-hua Zhou equally contributed to this study.

Abstract

Background: As the most predominant tumor-infiltrating immune cells, tumor-associated macrophages (TAMs) are associated with poor outcome in multiple solid cancers and play important roles in cancer progression. Cancer stem cells (CSCs) may account for metastasis and recurrence after cancer therapy. However, the association between TAMs and CSCs is not clarified in gastric cancer (GC). The aim of the present study was to evaluate the effects of TAMs on CSCs in GC and find out the risk factors to predict recurrence and prognosis.

Material and methods: This study included consecutive 236 patients with histologically confirmed primary GC. TAMs marker CD163 and CSCs-related proteins were detected by immunohistochemistry (IHC) in GC tissues and their prognostic values were all investigated.

Results: High expression of CD163+ TAMs was found in patents with aggressive characteristics, especially for patents with recurrence. There existed a significant correlation between high expression of CD163 and CSCs-related markers in GC tissues. In patients with recurrence, high-expression of CD163 TAMs was an independent worse prognostic factor.

Conclusion: High infiltration of TAMs was related to aggressive behavior, associated with aberrant expression of CSC markers, and an independent worse prognostic factor in GC. Targeting TAMs may be a potential treatment strategy for GC, including patients with recurrence.

Keywords: Gastric cancer, Tumor-associated macrophages (TAMs), Cancer stem cells (CSCs), Recurrent, Prognosis.

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How to cite this article:
Zhang Wj, Zhou Zh, Guo M, Yang Lq, Xu Yy, Pang Th, Gao St, Xu Xy, Sun Q, Feng M, Wang H, Lu Cl, Wu Gz, Guan Wx, Xu Gf. High Infiltration of Polarized CD163+ Tumor-Associated Macrophages Correlates with Aberrant Expressions of CSCs Markers, and Predicts Prognosis in Patients with Recurrent Gastric Cancer. J Cancer 2017; 8(3):363-370. doi:10.7150/jca.16730. Available from http://www.jcancer.org/v08p0363.htm