J Cancer 2017; 8(10):1750-1758. doi:10.7150/jca.18943

Research Paper

Knockdown of Tubulin Polymerization Promoting Protein Family Member 3 inhibits cell proliferation and invasion in human colorectal cancer

Kuanping Ye1*, Yintao Li2,3*, Weiwei Zhao4, Nan Wu5, Naijia Liu1, Rumei Li1, Lili Chen1, Min He1, Bin Lu1, Xuanchun Wang1, Renming Hu1✉

1. Department of Endocrinology and Metabolism, Institute of Endocrinology and Diabetology, Huashan Hospital, Fudan University, Shanghai, P.R. China
2. Department of Medical Oncology, Shandong Cancer Hospital and Institute, Jinan, Shandong, P.R. China
3. School of Medicine, Shandong University, Jinan, P.R. China
4. Department of Integrated Therapy, Fudan University Shanghai Cancer Center, Shanghai, P.R. China
5. Department of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai, P.R. China
*These authors contributed equally to this work.

Abstract

Tubulin Polymerization Promoting Protein Family Member 3 (TPPP3), a member of the TPPP protein family, has been reported to play important roles in initiation and progression of human cancers. However, the expression and underlying function of TPPP3 in colorectal cancer (CRC) have not yet been fully clarified. In this study, the mRNA and protein levels of TPPP3 in 96 clinical CRC specimens were determined by RT-PCR and immunohistochemistry. The relation between TPPP3 expression and clinicopathologic characteristics and overall survival (OS) were evaluated. Further experiments showed that knockdown of TPPP3 inhibited cell proliferation, migration and invasion and induced cell apoptosis in vitro. In addition, TPPP3 silencing resulted in a decrease of angiogenesis and S phase fraction. Thus, our results suggested that TPPP3 played an important role in CRC progress and might serve as novel therapeutic target for CRC treatment.

Keywords: colorectal cancer, TPPP3, proliferation, invasion, apoptosis

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How to cite this article:
Ye K, Li Y, Zhao W, Wu N, Liu N, Li R, Chen L, He M, Lu B, Wang X, Hu R. Knockdown of Tubulin Polymerization Promoting Protein Family Member 3 inhibits cell proliferation and invasion in human colorectal cancer. J Cancer 2017; 8(10):1750-1758. doi:10.7150/jca.18943. Available from http://www.jcancer.org/v08p1750.htm