J Cancer 2018; 9(4):683-689. doi:10.7150/jca.22279

Research Paper

Clinical and Diagnostic Significance of Homer1 in hepatitis B virus-induced Hepatocellular Carcinoma

Ping Luo1, Xiaobo Feng2, Wei Jing3, Man Zhu1, Nandi Li1, Hu Zhou1, paul F worley4, Hongyan Chai1✉, Jiancheng Tu1✉

1. Department of Clinical Laboratory Medicine & Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China;
2. Department of Pain, Zhongnan Hospital of Wuhan University, Wuhan 430071, China;
3. Department of Clinical Laboratory, First Affiliated Hospital of Zhengzhou University, Henan, 450000, China.
4. Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Abstract

Background: Hepatocellular carcinoma (HCC) is a malignant tumor worldwide. Attributed to the lack of early diagnosis index, most patients are diagnosed in their late stage. Homer1, as a member of scaffold protein family, is made up of two different isoforms: Homer1a and Homer1b/c. More and more evidences show that Homer1 is dysregulated in cancers. Here, in this study, we investigated the expression profile, clinical, diagnostic and prognostic significance of Homer1 in hepatitis B virus-induced HCC (HBV-HCC).

Methods: We first tested the expression of Homer1 in HCC cell lines by quantitative real-time PCR (qRT-PCR), western blot. Then, 86 pairs of tumorous and adjacent normal tissues from HCC together with a total number of 245 peripheral blood samples were enrolled to check the expression levels of Homer1 by quantitative real-time PCR (qRT-PCR).

Results: The results revealed that the levels of Homer1 were both downregulated in HCC cell line and tissue and were associated with tumor size, but were not related to the prognosis of HBV-HCC. Receiver-operating characteristic curve analyses indicated that the sensitivity of Homer1 to differentiate HCC patients from the controls was high to 100.0% and the combination of Homer1 and AFP got a higher prediction value of HCC (AUC=0.890).

Conclusion: Our data highlighted that Homer1 played a critical role in HCC tumorigenesis and might be a potential diagnostic marker for HCC.

Keywords: Homer1, HCC, Tumorigenesis, Diagnosis: Down-regulated

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Luo P, Feng X, Jing W, Zhu M, Li N, Zhou H, worley pF, Chai H, Tu J. Clinical and Diagnostic Significance of Homer1 in hepatitis B virus-induced Hepatocellular Carcinoma. J Cancer 2018; 9(4):683-689. doi:10.7150/jca.22279. Available from http://www.jcancer.org/v09p0683.htm