J Cancer 2019; 10(4):1032-1038. doi:10.7150/jca.24314
Eosinophil peroxidase over-expression predicts the clinical outcome of patients with primary lung adenocarcinoma
1. Department of Respiratory Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, China
2. Department of Respiratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China
3. Department of Respiratory Medicine, Jinling Hospital, Southern Medical University, Nanjing 210002, China
#These authors contributed equally to this work.
Eosinophil peroxidase (EPO), a heme protein abundantly expressed in eosinophils, involves in the catalysis of cytotoxic oxidants associated with the pathogenesis of cancer, asthma, and allergic inflammatory disorders. To date, its roles in the pathogenesis of lung cancer are still not known. We determined the expression of EPO in the lung adenocarcinoma tissues and the normal adjacent lung tissues using Real-time PCR and Western blotting analysis, respectively. Also, EPO protein expression in 90 lung adenocarcinoma (AD) samples were confirmed with immunohistochemistry (IHC) using tissue microarrays. Meanwhile, we investigated the association between EPO and the clinicopathological characteristics and disease prognosis in the pulmonary adenocarcinoma patients, which demonstrated that EPO mRNA and protein were significantly higher in lung AD tissues that those of the adjacent normal lung tissues (P<0.05). EPO overexpression was significantly correlated with pathologic-tumour nodes metastasen stage (p-TNM stage, P=0.017) and lymph node metastasis (P=0.027). Patients with EPO overexpression showed shorter survival time than those with low EPO levels (P=0.017), according to the Kaplan-Meier survival curve. Furthermore, a multivariate Cox regression model was utilized to analyze the prognostic factors, which indicated that N stage (HR=0.965, 95% CI=0.328-1.359, P=0.008), p-TNM Stage (HR=3.127, 95% CI =2.463-5.015, P=0.021) and high EPO protein expression (HR=3.145, 95% CI=2.016-5.519, P=0.018) were independent factors for the prognosis of lung AD. In conclusion, increased EPO expression could be used as a biomarker for lung AD patients with poor prognosis.
Keywords: Eosinophil peroxidase, adenocarcinoma, oxidative stress, prognosis
Ye L, Wang H, Li H, Liu H, Lv T, Song Y, Zhang F. Eosinophil peroxidase over-expression predicts the clinical outcome of patients with primary lung adenocarcinoma. J Cancer 2019; 10(4):1032-1038. doi:10.7150/jca.24314. Available from http://www.jcancer.org/v10p1032.htm