J Cancer 2019; 10(20):4902-4912. doi:10.7150/jca.29958

Research Paper

TGFBI Promotes Tumor Growth and is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma

Bing-jie Wang1,2*, Kun-ping Chi3*, Ru-ling Shen4, Sai-wei Zheng1, Yang Guo5, Jian-feng Li5, Jian Fei5✉, Yuan He1✉

1. Department of Oral Medicine, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, 200072, China.
2. Department of Stomatology, Ningbo Yinzhou People's Hospital, Zhejiang 315040, China.
3. Department of Pathology, First people's Hospital of Yunnan Province, Yunnan, 650032, China.
4. Shanghai Laboratory Animal Research Center, Shanghai 201203, China.
5. School of Life Science and Technology, Tongji University, Shanghai 200082, China.
*These authors contribute equally to this study.

Abstract

Purpose: In a previous study, we found that transforming growth factor beta-induced (TGFBI) is a hub gene strongly associated with oral squamous cell carcinoma (OSCC), using gene chip meta-analysis and PPI network analysis. Thus, the present study was established to explore the role of TGFBI in the pathogenesis of OSCC and to define the underlying mechanisms.

Methods: The correlations between TGFBI expression and the clinicopathological features and prognosis of OSCC were analyzed. Then, TGFBI-knockout HSC-3 cell lines were constructed using the CRISPR/Cas9 system. Cell proliferation, migration, and invasion in vitro were determined by cell counting, CCK-8, colony formation, and Transwell assays. Moreover, a xenograft animal study was implemented to determine the tumorigenicity and metastatic ability associated with TGFBI in vivo. The genes and pathways differentially expressed after TGFBI knockout were determined using transcriptional sequencing and bioinformatics.

Results: TGFBI expression was significantly higher in OSCC than in normal tissue. Its high expression was also correlated with high stage and was predictive of poor prognosis, as we expected. Knockout of TGFBI inhibited cell proliferation and clone formation, and enhanced cell migration and invasion in vitro. Besides, the xenograft animal study showed that TGFBI knockout suppressed tumor growth and metastasis in vivo. Furthermore, transcriptome sequencing revealed that genes associated with cell proliferation, metastasis, and inflammatory responses exhibited a change of expression upon TGFBI knockout. GO and KEGG analyses indicated that the function of TGFBI is related to responses to bacteria and inflammatory responses.

Conclusions: TGFBI overexpression can promote OSCC and is associated with poor prognosis in OSCC patients. TGFBI knockout can inhibit cell proliferation and metastasis in vivo. TGFBI may alter cell responses to bacteria, which causes an imbalance in the immune inflammatory response and promotes the development of OSCC.

Keywords: TGFBI, OSCC, CRISPR/Cas9, transcriptional sequencing, proliferation, migration

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How to cite this article:
Wang Bj, Chi Kp, Shen Rl, Zheng Sw, Guo Y, Li Jf, Fei J, He Y. TGFBI Promotes Tumor Growth and is Associated with Poor Prognosis in Oral Squamous Cell Carcinoma. J Cancer 2019; 10(20):4902-4912. doi:10.7150/jca.29958. Available from http://www.jcancer.org/v10p4902.htm