J Cancer 2019; 10(20):4947-4953. doi:10.7150/jca.30286

Research Paper

Saikosaponin-d Increases the Radiosensitivity of Hepatoma Cells by Adjusting Cell Autophagy

Yin-Di Tian1#, Shuai Lin2#, Peng-Tao Yang3#, Ming-Hua Bai3, Ying-Ying Jin3, Wei-Li Min2, Hong-Bing Ma3✉, Bao-Feng Wang3✉

1. Department of Infectious Diseases, the Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China
2. Department of Surgical Oncology, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710004, China
3. Department of Radiation Therapy, Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an 710004, China
#These authors contribute equally to this work.

Abstract

Radiotherapy for liver cancer can affect the level of autophagy in cells, and effective autophagy regulation can increase the radiosensitivity of liver cancer cells.Saikosaponin-d (SSd) is an effective active ingredient extracted from traditional Chinese medicine Bupleurum. We have confirmed previously in vitro and in vitro experiments that SSd can significantly induce apoptosis of liver cancer cells, increase the radiosensitivity of liver cancer cells.This study explored the role of autophagy in SSd-mediated radiosensitivity of liver cancer cells. MTT and clone formation experiments showed that radiation can inhibit the proliferation of hepatoma cells and reduce the colony formation of hepatoma cells. After the addition of SSd, the inhibitory effect of radiation on the proliferation and clonal formation of hepatoma cells was further enhanced. However, the addition of the autophagy inhibitor chloroquine or mTOR agonist can partially reverse the inhibitory effect of the combined treatment of SSd with radiation on the proliferation of hepatoma cells. Similarly, transmission electron microscopy and laser confocal microscopy showed that after the addition of SSd, the number of radiation-induced autophagosomes increased significantly in hepatoma cells and the intervention of mTOR agonist can reduce the formation of autophagosomes in hepatoma cells.In addition,Western blot analysis presented that radiation significantly increased LC3-II levels. Especially when SSd is added, LC3-II levels is further increased. Our data indicate that SSd can inhibit the growth of liver cancer cells and enhance cell radiosensitivity by inducing autophagy formation.

Keywords: Saikosaponin-d, Radiation, Hepatocellular carcinoma, Autophagy.

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How to cite this article:
Tian YD, Lin S, Yang PT, Bai MH, Jin YY, Min WL, Ma HB, Wang BF. Saikosaponin-d Increases the Radiosensitivity of Hepatoma Cells by Adjusting Cell Autophagy. J Cancer 2019; 10(20):4947-4953. doi:10.7150/jca.30286. Available from http://www.jcancer.org/v10p4947.htm