Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis

Bao, Xinyue; Leng, Xin; Yu, Tianyu; Zhu, Junzheya; Zhao, Yunhan; Tenzindrogar,; Yang, Zhiluo; Wu, Shaobo; Sun, Qi

doi:10.7150/jca.91082

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J Cancer 2024; 15(3):825-840. doi:10.7150/jca.91082 This issue Cite

Research Paper

Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis

Xinyue Bao^1,†, Xin Leng^2,†, Tianyu Yu^1,†, Junzheya Zhu¹, Yunhan Zhao¹, Tenzindrogar¹, Zhiluo Yang¹, Shaobo Wu^1,✉, Qi Sun^1,✉

1. Department of General Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
2. Department of Urology, Affiliated Kunshan Hospital of Jiangsu University, Suzhou215300, China.
† These authors contributed equally to this work and should be considered co-first authors.
* They are co-corresponding authors.

Citation:

Bao X, Leng X, Yu T, Zhu J, Zhao Y, Tenzindrogar , Yang Z, Wu S, Sun Q. Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis. J Cancer 2024; 15(3):825-840. doi:10.7150/jca.91082. https://www.jcancer.org/v15p0825.htm

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Abstract

Background: CDCA5 has been reported as a gene involved in the cell cycle, however current research provides little details. Our goal was to figure out its functions and probable mechanisms in pan-cancer.

Methods: Pan-cancer bulk sequencing data and web-based analysis tools were applied to analyze CDCA5's correlations with the gene expression, clinical prognosis, genetic alterations, promoter methylation, alternative splicing, immune checkpoints, tumor microenvironment and enrichment. Real‑time PCR, cell clone formation assay, CCK-8 assay, cell proliferation assay, migration assay, invasion assay and apoptosis assay were used to evaluate the effect of CDCA5 silencing on colon cancer cell lines.

Results: CDCA5 is highly expressed in most tumors, which has been linked to a poor prognosis. Immune checkpoints analysis revealed that CDCA5 was associated with the immune gene CD276 in various tumors. Single-cell analysis showed that CDCA5 correlated with proliferating T cell infiltration in COAD. Enrichment analysis demonstrated that CDCA5 may modify cell cycle genes to influence p53 signaling. The examination of DLD1 cells revealed that CDCA5 increased the proliferation and blocked cell apoptosis.

Conclusion: This study contributes to the knowledge of the role of CDCA5 in carcinogenesis, highlighting the prognostic potential and carcinogenic involvement of CDCA5 in pan-cancer.

Keywords: CDCA5, pan-cancer analysis, biomarker, prognosis, TCGA

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APA

Bao, X., Leng, X., Yu, T., Zhu, J., Zhao, Y., Tenzindrogar, , Yang, Z., Wu, S., Sun, Q. (2024). Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis. Journal of Cancer, 15(3), 825-840. https://doi.org/10.7150/jca.91082.

ACS

Bao, X.; Leng, X.; Yu, T.; Zhu, J.; Zhao, Y.; Tenzindrogar, ; Yang, Z.; Wu, S.; Sun, Q. Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis. J. Cancer 2024, 15 (3), 825-840. DOI: 10.7150/jca.91082.

NLM

CSE

Bao X, Leng X, Yu T, Zhu J, Zhao Y, Tenzindrogar , Yang Z, Wu S, Sun Q. 2024. Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis. J Cancer. 15(3):825-840.

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