CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis

Zhang, Peng; Yang, Qian; Chen, Xulong; Chen, Xiaolong; Wang, Qing; Chen, Kun; An, Yu; Jiang, Kehua; Sun, Fa

doi:10.7150/jca.90449

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International Journal of Medical Sciences

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Journal of Genomics

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J Cancer 2024; 15(3):858-870. doi:10.7150/jca.90449 This issue Cite

Research Paper

CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis

Peng Zhang^1,2#, Qian Yang^3#, Xulong Chen¹, Xiaolong Chen², Qing Wang², Kun Chen⁴, Yu An⁵, Kehua Jiang^2✉, Fa Sun^1,2✉

1. Guizhou Medical University, Guiyang, China.
2. Department of Urology, Guizhou Provincial People's Hospital, Guiyang, China.
3. Department of Gastroenterology, Guizhou Provincial People's Hospital, Guiyang, China.
4. Department of Medical Genetics, Guizhou Provincial People's Hospital, Guiyang, China.
5. NHC Key Laboratory of Pulmonary Immune-Related Diseases, Guizhou Provincial People's Hospital, Guiyang, China.
#Contributed equally to this work.

Citation:

Zhang P, Yang Q, Chen X, Chen X, Wang Q, Chen K, An Y, Jiang K, Sun F. CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis. J Cancer 2024; 15(3):858-870. doi:10.7150/jca.90449. https://www.jcancer.org/v15p0858.htm

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Abstract

Purpose: The objective of this study was to examine the expression and role of Centromere protein W (CENPW) in bladder cancer (BLCA), as well as its potential mechanistic impact on the progression of BLCA.

Methods: In this study, we conducted a comparative analysis of the mRNA expression level of CENPW in BLCA tissues and adjacent normal tissues using data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Additionally, we investigated the association between CENPW expression and patient prognosis. Furthermore, we performed in vitro and in vivo experiments to assess the impact of CENPW knockdown on various tumor biological phenotypes in BLCA. Finally, we conducted an analysis to elucidate the underlying mechanisms responsible for the observed phenotypic alterations in BLCA.

Results: The expression of CENPW was found to be upregulated in BLCA, and its higher expression was associated with a poorer disease-specific survival (DSS). CENPW was found to have close associations with the cell cycle, mitosis, and DNA replication. In vitro and in vivo experiments demonstrated that the inhibition of CENPW led to a suppression of BLCA progression. Specifically, the knockdown of CENPW resulted in cell cycle arrest phase and induced apoptosis in BLCA by potentially inactivating the signal transducer and activator of transcription3 (STAT3) signaling pathway.

Conclusion: CENPW has the potential to function as a molecular marker indicating an unfavorable prognosis in BLCA. Additionally, CENPW exhibits promise as a novel therapeutic target for BLCA.

Keywords: Bladder cancer, Disease-specific survival, Progression, Cell cycle, Apoptosis

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APA

Zhang, P., Yang, Q., Chen, X., Chen, X., Wang, Q., Chen, K., An, Y., Jiang, K., Sun, F. (2024). CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis. Journal of Cancer, 15(3), 858-870. https://doi.org/10.7150/jca.90449.

ACS

Zhang, P.; Yang, Q.; Chen, X.; Chen, X.; Wang, Q.; Chen, K.; An, Y.; Jiang, K.; Sun, F. CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis. J. Cancer 2024, 15 (3), 858-870. DOI: 10.7150/jca.90449.

NLM

CSE

Zhang P, Yang Q, Chen X, Chen X, Wang Q, Chen K, An Y, Jiang K, Sun F. 2024. CENPW knockdown inhibits progression of bladder cancer through inducing cell cycle arrest and apoptosis. J Cancer. 15(3):858-870.

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