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J Cancer 2024; 15(4):966-980. doi:10.7150/jca.89135 This issue Cite

Research Paper

n6-methyladenosine-modified circular RNA family with sequence similarity 126, member A affects cholesterol synthesis and malignant progression of prostate cancer cells by targeting microRNA-505-3p to mediate calnexin

Lin Luo1#, Ping Li1#, QingZhi Xie1, YunChou Wu1, FuQiang Qin1, DunMing Liao1, Ke Zeng1, KangNing Wang1,2✉

1. Department of Urology Surgery, The First Affiliated Hospital of Shaoyang University, Shaoyang City, Hunan Province, 422000, China.
2. Department of Urology Surgery, Xiangya Hospital Central South University, Changsha City, Hunan Province, 410083, China.
# These authors contributed equally to this work.

✉ Corresponding author: KangNing Wang, Department of Urology Surgery, The First Affiliated Hospital of Shaoyang University, No.39, Tongheng Street, Shuangqing District, Shaoyang City, Hunan Province, 422000, China, Email: csuxywknedu.cn.

Citation:
Luo L, Li P, Xie Q, Wu Y, Qin F, Liao D, Zeng K, Wang K. n6-methyladenosine-modified circular RNA family with sequence similarity 126, member A affects cholesterol synthesis and malignant progression of prostate cancer cells by targeting microRNA-505-3p to mediate calnexin. J Cancer 2024; 15(4):966-980. doi:10.7150/jca.89135. https://www.jcancer.org/v15p0966.htm
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Abstract

Graphic abstract

Prostate cancer (PCa) is the most commonly diagnosed malignancy in men. In tumor biology, n6-methyladenosine (m6A) can mediate the production of circular RNAs (circRNAs). This study focused on the mechanism of m6A-modified circRNA family with sequence similarity 126, member A (FAM126A) in PCa. Cell counting kit-8 assay, colony formation assay, 5-ethynyl-2'-deoxyuridine assay, transwell assay, and xenograft mouse models were applied to study the role of circFAM126A in PCa cell growth and tumor metastasis, and cellular triglyceride and cholesterol levels were measured to assess cholesterol synthesis. RNA immunoprecipitation, RNA pull-down, luciferase reporter gene assay, and western blot were adopted to explore the underlying molecular mechanism. Data showed that circFAM126A was upregulated in PCa and promoted PCa progression in vitro. m6A modification of circFAM126A enhanced transcriptional stability. CircFAM126A targeted microRNA (miR)-505-3p to mediate calnexin (CANX). Up-regulating miR-505-3p or inhibiting CANX suppressed cholesterol synthesis and malignant progression in PCa cells. Overexpressing CANX suppressed the inhibitory effect of circFAM126A silencing or miR-505-3p upregulation on PCa cells. Our current findings provide a new therapeutic strategy for the treatment of PCa.

Keywords: m6A, circFAM126A, miR-505-3p, Prostate cancer

Citation styles

APA
Luo, L., Li, P., Xie, Q., Wu, Y., Qin, F., Liao, D., Zeng, K., Wang, K. (2024). n6-methyladenosine-modified circular RNA family with sequence similarity 126, member A affects cholesterol synthesis and malignant progression of prostate cancer cells by targeting microRNA-505-3p to mediate calnexin. Journal of Cancer, 15(4), 966-980. https://doi.org/10.7150/jca.89135.

ACS
Luo, L.; Li, P.; Xie, Q.; Wu, Y.; Qin, F.; Liao, D.; Zeng, K.; Wang, K. n6-methyladenosine-modified circular RNA family with sequence similarity 126, member A affects cholesterol synthesis and malignant progression of prostate cancer cells by targeting microRNA-505-3p to mediate calnexin. J. Cancer 2024, 15 (4), 966-980. DOI: 10.7150/jca.89135.

NLM
Luo L, Li P, Xie Q, Wu Y, Qin F, Liao D, Zeng K, Wang K. n6-methyladenosine-modified circular RNA family with sequence similarity 126, member A affects cholesterol synthesis and malignant progression of prostate cancer cells by targeting microRNA-505-3p to mediate calnexin. J Cancer 2024; 15(4):966-980. doi:10.7150/jca.89135. https://www.jcancer.org/v15p0966.htm

CSE
Luo L, Li P, Xie Q, Wu Y, Qin F, Liao D, Zeng K, Wang K. 2024. n6-methyladenosine-modified circular RNA family with sequence similarity 126, member A affects cholesterol synthesis and malignant progression of prostate cancer cells by targeting microRNA-505-3p to mediate calnexin. J Cancer. 15(4):966-980.

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