J Cancer 2017; 8(7):1249-1254. doi:10.7150/jca.18230

Research Paper

Impact Of Circulating T Helper 1 And 17 Cells in the Blood on Regional Lymph Node Invasion in Colorectal Cancer

Ji Yeon Lee1*, Eun-Hye Seo1*, Chung-Sik Oh2, Jin-Hee Paik3, Dae-Yong Hwang3, Seung Hyun Lee1, Seong-Hyop Kim2, 4✉

1. Department of Microbiology, Konkuk University School of Medicine, Seoul, Korea;
2. Department of Anesthesiology and Pain medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea;
3. Department of Surgery, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea;
4. Department of Medicine, Institute of Biomedical Science and Technology, Konkuk University School of Medicine, Seoul, Korea.
* The two authors evenly contributed to the study as first author.

Abstract

We hypothesised that the blood levels of immune cells would be related to the progression of colorectal cancer and regional lymph node metastasis. We investigated the association between the blood levels of immune cells and regional lymph node metastasis in colorectal cancer patients. Patients with American Joint Committee on Cancer (AJCC) stages 1 and 2 colorectal cancer were assigned to Early stage group and those with AJCC stages 3 and 4 were assigned to Late stage group. Blood levels of circulating immune cells, such as cluster of differentiation (CD)4+ including T helper 1 (Th1) and 17 (Th17) cells, regulatory T (Treg) cells, CD8+ T cells, and natural killer (NK) cells were assessed using fluorescence-activated cell sorting (FACS). The blood levels of CD4+ T, Treg, CD8+ T, and NK cells did not significantly differ between the two groups. However, the blood levels of Th1 and Th17 cells did significantly differ between the groups. Specifically, Late stage group had higher levels of Th1 and Th17 cells than Early stage group (Th1, 11.14±1.22% vs. 16.25±1.57%, p = 0.015; Th17, 3.32±0.05% vs. 1.11±0.15%, p < 0.01). In conclusion, the blood levels of Th1 and Th17 cells significantly increased as the N stage increased. The blood levels of Th1 and Th17 cells might be useful as predictive markers of lymph node invasion in colorectal cancer.

Keywords: immune cell, lymph node metastasis, colorectal cancer, helper T cell.

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How to cite this article:
Lee JY, Seo EH, Oh CS, Paik JH, Hwang DY, Lee SH, Kim SH. Impact Of Circulating T Helper 1 And 17 Cells in the Blood on Regional Lymph Node Invasion in Colorectal Cancer. J Cancer 2017; 8(7):1249-1254. doi:10.7150/jca.18230. Available from http://www.jcancer.org/v08p1249.htm