MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3

Zhang, Jia Wei; Wang, Xing; Li, Gao Chao; Wang, Dong; Han, Sheng; Zhang, Yao Dong; Luo, Chen Huan; Wang, Hong Wei; Jiang, Wang Jie; Li, Chang Xian; Li, Xiang Cheng

doi:10.7150/jca.41437

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International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

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J Cancer 2020; 11(12):3604-3614. doi:10.7150/jca.41437 This issue Cite

Research Paper

MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3

Jia Wei Zhang^*, Xing Wang^*, Gao Chao Li, Dong Wang, Sheng Han, Yao Dong Zhang, Chen Huan Luo, Hong Wei Wang, Wang Jie Jiang, Chang Xian Li^✉, Xiang Cheng Li^✉

Hepatobiliary Center, The First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Nanjing, Jiangsu Province, China
*Equal contributors

Citation:

Zhang JW, Wang X, Li GC, Wang D, Han S, Zhang YD, Luo CH, Wang HW, Jiang WJ, Li CX, Li XC. MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3. J Cancer 2020; 11(12):3604-3614. doi:10.7150/jca.41437. https://www.jcancer.org/v11p3604.htm

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Abstract

Background: MicroRNAs (miRNAs) play important roles in the occurrence and development of cancers. In this project, we aimed to explore the role and molecular mechanism of mir-30a-5p in cholangiocarcinoma (CCA).

Materials and Methods: The expression profile and clinical significance of miR-30a-5p in CCA patients were investigated in 31 ICC and 52 ECC patients respectively. The role and mechanism of miR-30a-5p in CCA cells were investigated by up-regulating and inhibiting miR-30a-5p expression in vitro functional study.

Results: The expression of miR-30a-5p was increased in both CCA tissues and cells. The inhibition of miR-30a-5p decreased cell proliferation and induced cell apoptosis while overexpression of miR-30a-5p achieved the opposite effect. Furthermore, SOCS3 was down-regulated in ICC and ECC tissues and negatively regulated by miR-30a-5p. Dual-luciferase reporter assay revealed that co-transfection of miR-30a-5p significantly inhibited the activity of firefly luciferase reporter carrying the wild-type 3′UTR of SOCS3. The inhibition of SOCS3 could largely rescue the inhibitory effect of miR-30a-5p inhibition on CCA cells proliferation. In clinical, up-regulated miR-30a-5p expression was correlated with large tumor size in both ICC and ECC cohorts.

Conclusions: miR-30a-5p promoted CCA cells proliferation through targeting SOCS3. These findings suggested that miR-30a-5p could be a potential therapeutic target.

Keywords: miR-30a-5p, cholangiocarcinoma, SOCS3, proliferation, apoptosis

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APA

Zhang, J.W., Wang, X., Li, G.C., Wang, D., Han, S., Zhang, Y.D., Luo, C.H., Wang, H.W., Jiang, W.J., Li, C.X., Li, X.C. (2020). MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3. Journal of Cancer, 11(12), 3604-3614. https://doi.org/10.7150/jca.41437.

ACS

Zhang, J.W.; Wang, X.; Li, G.C.; Wang, D.; Han, S.; Zhang, Y.D.; Luo, C.H.; Wang, H.W.; Jiang, W.J.; Li, C.X.; Li, X.C. MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3. J. Cancer 2020, 11 (12), 3604-3614. DOI: 10.7150/jca.41437.

NLM

CSE

Zhang JW, Wang X, Li GC, Wang D, Han S, Zhang YD, Luo CH, Wang HW, Jiang WJ, Li CX, Li XC. 2020. MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3. J Cancer. 11(12):3604-3614.

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