J Cancer 2021; 12(12):3439-3447. doi:10.7150/jca.52118

Research Paper

Homer2 and Homer3 Act as Novel Biomarkers in Diagnosis of hepatitis B virus-induced Hepatocellular Carcinoma

Ping Luo1,6*, Chunzi Liang6*, Wei Jing2, Man Zhu3, Hu Zhou4, Hongyan Chai6, Paul F. Worley5, Jiancheng Tu6✉

1. Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, China.
2. Department of Clinical Laboratory, Key Laboratory of Laboratory Medicine of Henan, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China.
3. Department of Medical Laboratory, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China.
4. Department of Transfusion, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology.
5. Department of Neuroscience, School of Medicine, The Johns Hopkins University, Baltimore, MD 20205, USA.
6. Department & Program of Clinical Laboratory Medicine, Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
* These authors contributed equally

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Citation:
Luo P, Liang C, Jing W, Zhu M, Zhou H, Chai H, Worley PF, Tu J. Homer2 and Homer3 Act as Novel Biomarkers in Diagnosis of hepatitis B virus-induced Hepatocellular Carcinoma. J Cancer 2021; 12(12):3439-3447. doi:10.7150/jca.52118. Available from https://www.jcancer.org/v12p3439.htm

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Abstract

Background: Hepatocellular carcinoma (HCC) is one of the most common causes of cancer‐related mortality worldwide. Early detection of HCC can significantly improve patients' outcomes. An increasing number of studies have validated that Homer is dysregulated in cancers and may serve as diagnostic markers. In the present study, we investigated the expression profile and diagnostic significance of Homer2 and Homer3 in hepatitis B virus-induced HCC (HBV-HCC).

Methods: Quantitative real-time PCR (QRT-PCR), western blot analysis and immunohistochemistry analysis.

Results: Homer2 and Homer3 were downregulated in HCC. The expression of Homer2 was associated with tumor differentiation grade (P= 0.012) and total protein (TP) level (P= 0.032). Homer3 was related to tumor size (P= 0.010), tumor nodes (P= 0.026) and γ-glutamyl transferase (GGT) level (P= 0.001). The receiver operating characteristic curve analyses indicated that the combination of Homer2, Homer3 and AFP possessed a high accuracy (AUC=0.900) to diagnose HCC cases from healthy controls.

Conclusion: Our data indicated that Homer2 and Homer3 were downregulated in HCC and might be potential diagnostic marker for HCC.

Keywords: Homer2, Homer3, HCC, Tumorigenesis, Diagnosis.