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J Cancer 2017; 8(5):887-893. doi:10.7150/jca.17747 This issue Cite

Research Paper

Aided Diagnosis of Hepatocellular Carcinoma Using Serum Fucosylated Haptoglobin Ratios

Shuxin Shang^{1, 2}, Wei Li³, Xue Qin^2✉, Shu Zhang^1✉, Yinkun Liu^{1, 3}

1. Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Shanghai, China;
2. Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China;
3. Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.

✉ Corresponding authors: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China. Tel.: +86 21 54237962; Fax: +86 21 54237959 Email: qinxue919com (X. Qin); zhang.shush.cn (S. Zhang)

Abstract

Aberrant fucosylation plays a functional role in regulating ontogeny and celluar differentiation and are differentially regulated in cancerous condition, which could provide hallmarks for cancer diagnostics and surveillance. We previously developed a magnetic beads-based lectin ELISA system to measure fucosylated haptoglobin (Hp), which has been reported to be a cancer biomarker through a series of glycoproteomic analysis. In this study, serum fucosylated Hp ratios were measured using our ELISA kit in a separate cohort of 260 patients independently, including 130 healthy controls and 130 patients with hepatocellular carcinoma (HCC). Fucosylated Hp /Hp ratio (levels of fucosylated Hp /levels of protein Hp) and ELISA Index (OD value of fucosylated Hp /OD value of protein Hp) were calculated respectively to reflect Hp fucosylation level on its protein level. Our data showed that fucosylated Hp /Hp ratio (AUC=0.8449) and ELISA Index (AUC=0.8581) had better performance in distinguishing HCC from controls, which indicated that fucosylated Hp ratios could improve the diagnosis and prediction of HCC even with a low level of alpha-fetoprotein (AFP). Additionally, the combination analysis of AFP and fucosylated Hp ratios increased the AUC value for HCC diagnosis.

Keywords: biomarker, fucosylation, haptoglobin, hepatocellular carcinoma.

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