J Cancer 2017; 8(14):2854-2865. doi:10.7150/jca.18931 This issue Cite

Research Paper

MicroRNA-216b-5p Functions as a Tumor-suppressive RNA by Targeting TPT1 in Pancreatic Cancer Cells

Yu You*, Jiaxin Tan*, Yi Gong, Haisu Dai, Haowei Chen, Xuejun Xu, Aigang Yang, Yujun Zhang, Ping Bie

Department of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China
* These two authors contributed equally to this work

Citation:
You Y, Tan J, Gong Y, Dai H, Chen H, Xu X, Yang A, Zhang Y, Bie P. MicroRNA-216b-5p Functions as a Tumor-suppressive RNA by Targeting TPT1 in Pancreatic Cancer Cells. J Cancer 2017; 8(14):2854-2865. doi:10.7150/jca.18931. https://www.jcancer.org/v08p2854.htm
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Abstract

MicroRNAs (miRNAs) are increasingly recognized as being involved in pancreatic cancer progression by directly regulating the expression of their targets. In this study, we showed that miR-216b-5p expression was significantly decreased in pancreatic cancer tissues and cell lines. In addition, low miR-216b-5p expression was significantly associated with large tumor size and advanced TNM stage. Meanwhile, both Kaplan-Meier and multivariate survival analysis showed that decreased miR-216b-5p expression was associated with overall survival. miR-216b-5p over-expression repressed pancreatic cancer cell proliferation and induced cell cycle arrest and cell apoptosis in vitro and inhibited tumorigenesis in vivo. The translationally controlled tumor protein (TPT1) was identified as a novel direct target of miR-216b-5p. miR-216b-5p up-regulation suppressed TPT1 expression. Moreover, TPT1 mRNA expression levels were increased in pancreatic cancer tissues, and were inversely correlated with miR-216b-5p expression. TPT1 down-regulation had similar effects as miR-216b-5p up-regulation on pancreatic cancer cell progression. The restoration of TPT1 reversed the effect of miR-216b-5p on pancreatic cancer cell progression. Furthermore, we found that miR-216b-5p up-regulation suppressed Pim-3, Cyclin B1, p-Bad and Bcl-xL protein expression. However, the effect of miR-216b-5p up-regulation was partly reversed by TPT1 up-regulation in vitro. Taken together, our findings suggested that miR-216b-5p functions as a potential tumor suppressor by regulating TPT1 in pancreatic cancer cells, and it may represent a potential therapeutic target for patients with pancreatic cancer.

Keywords: miR-216b-5p, TPT1, pancreatic cancer, cell cycle.


Citation styles

APA
You, Y., Tan, J., Gong, Y., Dai, H., Chen, H., Xu, X., Yang, A., Zhang, Y., Bie, P. (2017). MicroRNA-216b-5p Functions as a Tumor-suppressive RNA by Targeting TPT1 in Pancreatic Cancer Cells. Journal of Cancer, 8(14), 2854-2865. https://doi.org/10.7150/jca.18931.

ACS
You, Y.; Tan, J.; Gong, Y.; Dai, H.; Chen, H.; Xu, X.; Yang, A.; Zhang, Y.; Bie, P. MicroRNA-216b-5p Functions as a Tumor-suppressive RNA by Targeting TPT1 in Pancreatic Cancer Cells. J. Cancer 2017, 8 (14), 2854-2865. DOI: 10.7150/jca.18931.

NLM
You Y, Tan J, Gong Y, Dai H, Chen H, Xu X, Yang A, Zhang Y, Bie P. MicroRNA-216b-5p Functions as a Tumor-suppressive RNA by Targeting TPT1 in Pancreatic Cancer Cells. J Cancer 2017; 8(14):2854-2865. doi:10.7150/jca.18931. https://www.jcancer.org/v08p2854.htm

CSE
You Y, Tan J, Gong Y, Dai H, Chen H, Xu X, Yang A, Zhang Y, Bie P. 2017. MicroRNA-216b-5p Functions as a Tumor-suppressive RNA by Targeting TPT1 in Pancreatic Cancer Cells. J Cancer. 8(14):2854-2865.

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