State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi, 330047, China.
# These authors contributed equally for this work.
Phytoestrogens are polyphenol compounds which have similar structure to 17β-estradiol (E2), a kind of main estrogen in women. Thus, phytoestrogens may affect the reproductive and endocrine systems, leading to the development of estrogen-related cancers. The effect of genistein (Gen), one of the most studied phytoestrogens, on human cervical cancer cells (HeLa) was investigated in this study. It was found that Gen at concentrations of 0.001, 0.01, 0.1 and 1 µmol·L-1 promoted the proliferation of HeLa cells in a dose-dependent manner. Gen increased the portion of HeLa cells in S phase and decreased the portion of the cells in G1 phase. Besides, apoptosis rate of the cells was significantly lower when treated with Gen compared with the control group. It was also found that the expression of ERα, Akt or nuclear NF-κB p65 protein was activated by Gen. The correlation between these three proteins may be as following: ERα was the upstream, followed by Akt, and then nuclear NF-κB p65 protein. In addition, the downstream genes of activated nuclear NF-κB p65 were found to be associated with cell cycle and apoptosis of cancer cells. Our results suggested that Gen may stimulate cell proliferation partially through the estrogen receptor-mediated PI3K/Akt-NF-κB pathway and the further activation of the downstream genes of nuclear NF-κB p65.
Keywords: Gen, Cell cycle, Cell apoptosis, ER, PI3K/Akt, nuclear NF-κB p65
Genistein (Gen), recognized as the predominant isoflavone in soybean enriched foods, is one of the most extensively studied phytoestrogens which are available in Asian diets . Epidemiological studies have provided evidence for a protective role of isoflavones against the development of numerous cancers including breast cancer, cervical cancer and prostate cancer . There were epidemiological data showing that Asian women have a significantly lower risk of breast cancer compared with Western women . Therefore, the supplement of the phytoestrogens like Gen has become prevalent in many countries in order to avoid the potential risk of estrogen-dependent cancers or alleviate the symptoms of menopause . However, the safety and efficacy of Gen intake were questioned, as some studies have shown that the concentration used by the researchers who found the inhibitory effect of Gen on cancers was higher than the level in the plasma , and the results about the effect of Gen on cancers were controversial . Therefore, the effect of Gen at lower doses on cancers should be considered.
The Protein Kinase B (Akt) pathway plays a critical role in regulating the balance between cell survival and apoptosis, as well as the proliferation, growth, migration and metabolism . Furthermore, the activation of Akt has been reported to be related to the pathogenesis of numerous disorders, including cancers . The main mechanism of Akt's action could be its ability of regulating the downstream node in either promoting or inhibiting ways . For instance, activated Akt can deliver anti-apoptotic signals by phosphorylating the Bad protein whose function was pro-apoptosis under normal circumstances . There was another situation that activated Akt can phosphorylate the p21Waf1/Cip1 and p27Kip1, two members of the cyclin-dependent kinase inhibitors, resulting in the promotion of the cell cycle. Akt can also affect the cell cycle through cyclin D1, which was significant in G1/S phase transition . Among all the downstream nodes, Nuclear Factor κB p65 (NF-κB p65) was another important signal node that needed to be taken into account. Under non-stimulating conditions, NF-κB p65 was combined with its inhibitor (IκB) in the cytoplasm, showing no function. Once stimulated, the IκB was degraded, and NF-κB p65 translocates to the nucleus and binds to the corresponding DNA-binding sites or interacts with other transcription factors, and then exerts transcriptional regulatory activity and anti-apoptosis function [12, 13]. In this study, the effect of Gen at lower doses on human cervical cancer and the possible signal transduction pathway involved were investigated.
The human cervical cancer cell line (HeLa) was obtained from American Type culture collection (ATCC). Gen (purity≥99%) and estrodiol (purity≥99%) were from Sigma (St Louis, MO, USA). Antibodies against ERα, Akt, NF-κB were from Cell Signaling (Beverly, MA, USA). FBS (Fetal bovine serum) and charcoal dextran-treated FBS (CDT-FBS) were from Hyclone (Logan, USA). Dulbecco's modified Eagle medium (DMEM) was obtained from Gibco (Grand Island, NY, USA). 3-[4, 5-dimethylthiazol 2-yl] 2, 5-diphenyltetrazolium bromide (MTT) was purchased from Solarbio (Beijing, China). Cell cycle and apoptosis analysis kits were from KeyGEN (Nanjing, China). Nucleoprotein and cytoplasm protein extraction kit (Nanjing China). Trizol was from Ambion (Texas, USA).
The HeLa cells were routinely cultured in DMEM supplemented with 10% FBS. The cells were maintained at 37 ºC in a humidified incubator with 5% CO2. Then the medium was changed to phenol red-free DMEM containing 5% charcoal dextran-treated FBS for 2 days before the experiment. The cells were treated with 0.001, 0.01, 0.1 and 1 μmol·L-1 Gen in the assay of MTT and cycle analysis.
The proliferation of HeLa cells was assessed by MTT assay. HeLa cells were seeded in 96-well plates at the initial density of 104 cells per well, and then incubated for 24 h before the treatment with Gen at different doses. After the treatment for 48 h, 20 μL MTT solution (5 mg·mL-1 in PBS) were added into each well, followed by further incubation for 4 h. The supernatant was discarded and 150 μL DMSO was added into each well to dissolve the formazan crystals produced. The absorbance was measured at 570 nm with a microplate reader. Cell proliferation rate was calculated from the value of the absorbance.
HeLa cells were seeded in 6-well plates at the density of 5×105 mL-1. Cells were incubated for 24 h before the treatment with Gen at different concentrations. After the treatment for 48 h, the cells were harvested by trypsinization, collected and centrifuged at 1000 rpm for 5 min. Then the cells were washed by ice-cold PBS twice, followed by fixing in ice-cold 70% ethanol at 4 ºC overnight. The ethanol was removed by centrifugation and the cells were washed with cold PBS. The fixed cells were then re-suspended with 100 μL RNase (0.6 mg·mL-1), incubated in the dark at 37 ºC for 30 min and then mixed with 400 μL propidium iodide (PI, 50 µg·mL-1), followed by the incubation at 37 ºC for 30 min. The cell cycle was then analyzed within one hour by flow cytometry.
The cells were seeded in 6-well plates at the density of 5×105 mL-1. The cells were harvested after treating with Gen for 48 h and washed by PBS twice. Then 500 μL Binding Buffer were added to re-suspend the cells. At last, 5 μL Annexin V-FITC and 5 μL Propidium Iodide were mixed well in the solution, followed by the incubation at room temperature for 10 min in the dark. The cell apoptosis should be assessed within 1 h by flow cytometry (BD FACSCaliburTM NewYork USA). WinMDI software was used to analyse the data.
HeLa cells were harvested after 0.1 μmol·L-1 Gen treatment for 0.5 h, 1 h, 2 h or 3 h. Cells were lysed, cytoplasmic and nuclear protein factions separated using the Nucleoprotein and Cytoplasm extraction kit, Key GEN BioTECH (Nanjing, China), following the procedure recommended by the manufacturer (or something similar). The protein was electrophoresed on 10% sodium dodecyl sulfate-polyacrylamide gels, and then transferred to nitrocellulose membrane. After bolcking by Bovine Serum Albumin, the membrane was incubated by corresponding antibody (diluted 1:1000) overnight at 4 ºC. Then the membrane was washed by TBST solution (containing 0.1% Tween 20 and 1% Tris) for 3 times (15 min each time). After washing, the anti-mouse or anti-rabbit IgG (diluted 1:10000) was added to the membranes for 1 h at the room temperature. The membrane was washed again by the TBST solution for 3 times (15 min each time). The proteins were visualized using enhanced chemiluminescence reagents. The signal was detected using a gel documentation system (Molecular Imager ChemiDocTM XRS+ Imaging Syste). The intensity of the signal was analyzed by Quantity One and expressed as the relative intensity compared to the control group (taken as 100%). β-actin was used as an internal control.
Reverse transcription-PCR (ABI Prism 7900HT, Applied Biosystems, USA) was used to detect the expression of Cyclin-D1 and Bcl/Bax genes. The reaction time of Gen were 0 h、3 h、6 h、12 h and 24 h, respectively. Total RNA was extracted from HeLa cells and then reverse transcribed into cDNA. The synthesized cDNA was used for PCR amplification. Primer sequences of selected genes were shown in Table 1. The PCR cycle was as following: initial denaturation at 94 ºC for 3 min, followed by denaturation at 94 ºC for 30 s, annealing at 60 ºC for 30 s and extension at 72 ºC for 2 min for 30 cycles, and 10 min at 72 ºC for final extension. The PCR products were separated by electrophoresis on a 2% agarose gel. β-actin was used as an internal control.
Data were expressed as means ± SD from at least three experiments. Statistical analyses for gene and protein expression were performed using the Quantity One Software. Statistical analyses for cell apoptosis were performed using the WinMDI software and the program used to analyze cell cycle phases was ModFit LT V3.2. All statistical analyses were performed using SPSS 13.0 statistical package (One-way Anova Tukey test). A value of *P<0.05 was considered to be significantly different. Origin 7.0 was used to draw column diagrams.
Incubation of HeLa cells with Gen at different concentrations (0.001, 0.01, 0.1 and 1 μmol·L-1) for 48 and 72 h significantly increased cell proliferation in a dose-dependent manner (Figure 1). The most significant proliferative effect on cell viability in comparison with the control group was achieved at the dose of 0.1 μmol·L-1 after 72 h treatment.
Primers used for RT-PCR
|Primer Sequence||Size of product||Tm (ºC)||Cycles|
Flow cytometry assay showed the distribution of cells in different phases of cell cycle. Analysis of cell cycle showed that treatment of cells for 48 h with Gen increased the percentage of cells in S phase and decreased the percentage of cells in G1 phase, and the number of cells in S phase was the highest at the concentration of 0.010 μmol·L-1 (Table 2).
The effect of Gen on cycle of HeLa cells
Difference was significant when *p< 0.05 vs Control Group (n=3)
It was found that treatment of Gen at all concentrations decreased the apoptosis rate of HeLa cells significantly as compared to the untreated cells (Table 3). The apoptosis rates of treated groups were (7.82%±1.73), (7.05%±2.86), (6.72%±1.92) and (6.73%±1.23) at 1, 0.1, 0.01 and 0.001 μmol·L-1, respectively.
To determine whether PI3K/Akt and nuclear NF-κB p65 signal transduction pathways were related to the proliferative effect of Gen on HeLa cells, expression levels of ERα, p-Akt and nuclear NF-κB p65 proteins were detected by western blotting. Gen at 0.1 μmol·L-1, the concentration which had the most significantly proliferative effect in the MTT assay, was used to treat the cells. As shown in Figure 2, the expression levels of ERα, p-Akt and nuclear NF-κB p65 of the Gen-treated group were much higher than the control group.
The effect of Gen on apoptosis of HeLa cells
Difference was significant when p< 0.05 vs Control Group; * is when p< 0.05 (vs Control Group) ; ** is when p< 0.01 (vs Control Group, n=3)
Besides, we also determined the cell viability when the cells were incubated with Gen and an inhibitor of ERα (MPP), p-Akt (LY294002) or nuclear NF-κB p65 (PDTC) to further determine whether Gen-induced proliferative effect was associated with the activation of these proteins. As shown in Figure 3, cell viabilities of the groups treated with both Gen and each inhibitor were significantly lower than the group treated with Gen alone. These results further confirm the relationship between the increased cell viability and activation of ERα, PI3K/Akt or nuclear NF-κB p65 protein.
The proliferative effect of different concentrations of Gen on HeLa cells. Gen groups were treated with increasing doses (0.001, 0.01, 0.1 and 1 μmol·L-1) for 48 h and 72 h. Cell proliferation index was obtained by the formula: PR% = (OD value of the Gen group/OD value of the control group) ×100%. Difference was significant when *p < 0.05 vs Control Group (n=3).
MPP and LY294002 are the specific inhibitors of ERα and PI3K/Akt respectively. To determine the connection between ERα and PI3K/Akt/NF-κB p65 pathway, the blocking ability of MPP and LY294002 was investigated. As shown in Figure 4, expression level of p-Akt was lower when the cells were treated with MPP. Furthermore, expression level of nuclear NF-κB p65 was also lower after treating with LY294002. These results indicated that ERα was activated before PI3K/Akt, followed by the activation of NF-κB p65.
Gen was found to activate nuclear NF-κB which has the ability of regulating the expression of several cell cycle and apoptosis related genes. Therefore, we determined (urokinase type plasminogen activator), TRAF (tumour-necrosis factor receptor associated factor)-1, and c-IAP-1/2 (two inhibitors of apoptosis family). As shown in Figure 5, gene expression level of Cyclin D1 increased, which could explain the shortening of G1 phase in the cells. The increased gene expression levels of Bcl-2, VEGF, u-PA and TRAF-1, and the decreased gene expression level of c-IAP-1 could explain the reduction of apoptosis rate of the cells. In addition, the stable expression of c-IAP-2 suggested that c-IAP-2 may not be associated with apoptosis of the cells.
Cervical cancer is one of the leading causes of death in women around the world. Previous studies have found the therapeutic effect of phytoestrogen on cervical cancer. However, the concentrations (above 1 μmol·L-1) used in the therapy were much higher than the plasma level in healthy people and its content in the diets [14, 15, 16]. It has been reported that phytoestrogen can not only exert the anti-estrogenic effect (at high concentrations), but also the estrogenic effect (at low concentrations) . Therefore, taking the average dietary intake of Gen into account, the study on the effect of Gen at lower concentrations was of more practical significance. In the present study, we thus investigated the estrogenic effect of Gen at 0.001 μmol·L-1 to 1 μmol·L-1 on human cervical cancer in vitro, and the possible molecular mechanism of its action.
A : The expression of ERα、p-Akt and nuclear NF-κB p65 proteins of HeLa cells treated with Gen; B: The effect of Gen on the expression of ERα protein. *p<0.05 vs control group (t=0), n=3. C: The effect of Gen on the expression of p-Akt protein. *p<0.05 vs control group (t=0), n=3. D: The effect of Gen on the expression of nuclear NF-κB p65 protein.*p<0.05 vs control group (t=0), n=3. Gen induced activation of ERα, PI3K/Akt and nuclear NF-κB p65 proteins in HeLa cells. HeLa cells were treated with Gen (0.1 μmol‧L-1) for increasing time points (0, 0.5, 1, 2 and 3 h). Time course study of protein expression of ERα、p-Akt and nuclear NF-κB p65 by Western blotting.
Previously studies shown that Gen could inhibit cell proliferation and induce apoptosis: Yang et al. reported that Gen (25, 50, 100 μmol·L-1) suppressed the viability of HeLa cells, caused apoptosis in a dose dependent manner and ER stress contributes to Gen-induced apoptosis in cervical cancer cells . Ouyang et al. reported that exposure of human ovarian cancer HO-8910 cells to Gen (100 µmol·L-1) could induce DNA damage, triggers G2/M phase arrest and cell apoptosis . While according to our study the effect of Gen on HeLa cells were totally different when treated HeLa cells with low concentrations of Gen (0.001-1 µmol·L-1). We determined whether Gen could exert proliferative effect on HeLa cells by examining cell viability, cell cycle distribution by MTT assay and flow cytometry respectively. The results showed that Gen promoted cell proliferation and enhanced the portion of cells in S phase. We then investigated whether Gen might also affect the apoptosis of HeLa cells as apoptosis plays an important role in maintaining cell homeostasis. These results suggested that Gen may influence cell proliferation through its ability of regulating cell cycle and apoptosis.
Inhibitory effect of MPP, LY294002 and PDTC (25 μmol·L-1) on viability of HeLa cells treated with Gen at 0.1 μmol·L-1, (n=3). Cells were incubated with Gen and each inhibitor for 24 h. Difference was significant when *p < 0.05 vs Control Group (inhibitor = 0, defined as 100%).
The correlation determination between the ERα, p-Akt and nuclear NF-κB p65. A: Cells of three groups were incubated for 1 h. Then the expression levels of p-Akt protein was analyzed by western blotting. B: Cells of three groups were incubated for 1 h. Then the expression of nuclear NF-κB p65 protein was detected by western blotting.
The detection of the related downstream genes of NF-κB. Some downstream genes of NF-κB, Cyclin D1, Bcl-2, VEGF, u-PA, TRAF-1, c-IPA-1 and c-IPA-2 were detected. The cells were treated with Gen for increasing time points (0, 3, 6, 12 and 24 h). The gene expression was analysed by RT-PCR
E2 could bind to ER and trigger ER-mediated responses . Gen had similar chemical structure to E2, thus allowing it competitively bind to ER . To determine whether Gen could activate the estrogenic pathway, we analyzed the expression of ERα in HeLa cells. It was found that expression of ERα protein significantly increased as compared with the control group. Besides, we used MPP (an inhibitor of ER) to block the ER pathway, and found that the proliferative effect of Gen has been weakened to a certain extent, but not entirely. This result indicated that Gen may exert its proliferative effect partially through ER-mediated pathway, and there might be other pathways involved here.
The development of cancers is a complex process which involves various factors and pathways. Within these factors, there are many connections and crosstalks between them. For instance, the upstream proteins can activate downstream proteins by phosphorylation and activated protein might have the ability of regulating some related genes which can regulate other proteins in turn . Eventually, it would generate a series of comprehensive responses. In this study, we mainly focused on the PI3K/Akt pathway because of its important role in controlling the balance between cell survival and apoptosis, especially in cancer progression. The results showed that expression levels of p-Akt protein in Gen-treated groups were much higher as compared with the control group. These results suggested that PI3K/Akt pathway was indeed activated by Gen. In addition, the cell viability was weakened by LY294002, which has the ability of inhibiting Akt activity specifically. It was concluded from these results that PI3K/Akt pathway might be associated with the proliferative effect induced by Gen. Then, among all the downstream proteins of Akt, we further determined the expression of nuclear NF-κB p65 because it was an important nuclear transcription factor that could regulate cycle and apoptosis-related genes and also has been reported to be involved in estrogen response pathway . It was found that nuclear NF-κB p65 protein expression level was significantly increased by Gen. Furthermore, blocking of nuclear NF-κB p65 by its inhibitor PDTC was found to weaken the cell viability, suggesting the correlation between the nuclear NF-κB p65 and the proliferative effect of Gen.
NF-κB has been reported to influence the development and metastasis of tumor because of its ability of regulating many related genes . In order to explore the role of Gen on cell cycle and apoptosis, we selected several downstream genes of NF-κB which were related to the regulation of cell cycle or apoptosis. Cyclin D1, a regulator of cell cycle, exerts its role with cyclin-dependent kinases and their inhibitors, whose contents change with the cell cycle. The overexpression of Cyclin D1 was characteristic of a variety of human tumors. Some studies have suggested that its overexpression could shorten the G1 phase, leading to cell proliferation [25-28]. Bcl-2 was one of the members in Bcl-2 family which were associated with apoptosis. When Bcl-2 was relatively excessive, cells can avoid apoptosis . VEGF was a cytokine secreted by malignancies. It has the strongest effect on promoting angiogenesis among all the pro-angiogenic regulatory factors, which can promote tumor angiogenesis, thus contributing to tumor growth and progression . Activated u-PA can promote the degradation of extracellular matrix, and then the tumor invasion and metastasis can be achieved [31, 32]. TRAF-1 and c-IAP1/2 were two main factors that were associated with the apoptosis like the Bcl-2 family . The RT-PCR results showed that these genes were indeed up-regulated or down-regulated. And the variation of these genes was in accordance with the change of cell cycle and apoptosis rate of HeLa cells, which could further explain the proliferation of HeLa cells.
Gen triggered the activation of ER, PI3K/Akt and NF-κB pathways one after another, and then regulated the expression of related genes through activation of NF-κB, resulting in the variation of cell cycle and apoptosis and leading to the proliferative effect eventually. The results reflected the effect of these proteins on the proliferation of HeLa cells. However, there might be other pathways through which Gen exerted its function because blocking of this pathway did not suppress the promoting effect entirely. Overall, the study has demonstrated the proliferative effect of Gen on HeLa cells, the important role of ER, PI3K/Akt or NF-κB in this effect, and the possible reasons of causing this effect. The results may provide some experimental and theoretical basis for the necessity of being cautious about the intake of phytoestrogen and the use of phytoestrogen in cancer therapy.
Gen, genistein; Akt, Protein Kinase B; E2, 17β-estradiol; ERα, estrogen receptor α; I-κB, inhibitors of κB; NF-κB, Nuclear Factor κB; TRAF-1, tumour-necrosis factor receptor associated factor-1; u-PA, urokinase type plasminogen activator; VEGF, vascular endothelial growth factor.
The financial support for this study by Joint Sino-German Research Project of National Natural Science Foundation of China (No.GZ 731), International Science & Technology Cooperation Program of China (No.2010DFA31780), National Natural Science Foundation of China Used to Support regional science fund (NO.81460468), Jiangxi Youth Science Foundation Program (NO. 20142BAB215044),is gratefully acknowledged.
S.P. N., M.Y. X., W.J. L. designed research; H.H. C., S.P. C., Q.L. Z. performed research and analyzed data, H.H. C., S.P. C.,X.J. H. wrote the paper.
The authors have declared that no competing interest exists.
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Corresponding authors: Professor Ming-Yong Xie, PhD; Professor Shao-Ping Nie, PhD; Tel/Fax: +86-791-83969009 (M. Y. XIE); Tel/Fax: +86-791-88304452 (S. P. NIE); E-mail address: myxieedu.cn; spnieedu.cn