J Cancer 2018; 9(15):2603-2611. doi:10.7150/jca.24918 This issue

Research Paper

microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147

Xiu-Xiu Hu1,2#, Xue-Ni Xu1,2#, Bang-Shun He2, Hui-Ling Sun2, Tao Xu2, Xiang-Xiang Liu2, Xiao-Xiang Chen1,2, Kai-Xuan Zeng1,2, Shu-Kui Wang1,2✉, Yu-Qin Pan2✉

1. Medical School of Southeast University, Nanjing, Jiangsu, China
2. General Clinical Research Center, Nanjing First Hospital, Nanjing Medical University, Nanjing 210000, China
#These two authors contributed equally to this paper.

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Hu XX, Xu XN, He BS, Sun HL, Xu T, Liu XX, Chen XX, Zeng KX, Wang SK, Pan YQ. microRNA-485-5p Functions as a Tumor Suppressor in Colorectal Cancer Cells by Targeting CD147. J Cancer 2018; 9(15):2603-2611. doi:10.7150/jca.24918. Available from https://www.jcancer.org/v09p2603.htm

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Colorectal cancer(CRC) is a prevalent malignancy in the world. There is growing evidence that microRNAs (miRNAs) as crucial modulator are in connection with many tumor-related diseases including CRC. Though miR-485-5p has been reported as an anti-oncogene in certain cancers, it remains unclear in CRC. In this research, we found that miR-485-5p was at lower level expression in CRC tissues and cell lines compared to the paired paracancerous tissues and the normal colon epithelial cell line FHC, correspondingly. Furthermore, Experimental up-regulation miR-485-5p in DLD-1 and SW480 cells with mimic could inhibit the ability of proliferation, migration, invasion of CRC cell lines and facilitate cells apoptosis. Also, we confirmed that CD147 existed typically negative regulation by miR-485-5p through binding a conserved sequence specifically within the CD147 3′-untranslated region (3′UTR) and reintroduction of CD147 could rescue the phenotypic changes caused by miR-485-5p. The findings provide evidence to demonstrate the role of miR-485-5p/CD147 interaction in CRC and indicate that miR-485-5p might be exploited therapeutically in CRC.

Keywords: miR-485-5p, colorectal cancer, proliferation, migration, invasion, CD147