J Cancer 2018; 9(17):2994-3005. doi:10.7150/jca.25876 This issue

Research Paper

Loss of UHRF2 Is Associated With Non-small Cell Lung Carcinoma Progression

Chun Jin1*, Dian Xiong2*, Hao-Ran Li1*, Jia-Hao Jiang1, Jian-Chao Qi3, Jian-Yong Ding1✉

1. Department of Thoracic Surgery, The Affiliated Zhongshan Hospital of Fudan University, Shanghai 200032, P. R. China
2. Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Nanchang University, Jiangxi Province 330000, P. R. China.
3. Department of emergency surgery, Fujian Provincial Hospital, Fu Zhou, Fujian Province,350001, China
* These authors contributed equally to this work.

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Jin C, Xiong D, Li HR, Jiang JH, Qi JC, Ding JY. Loss of UHRF2 Is Associated With Non-small Cell Lung Carcinoma Progression. J Cancer 2018; 9(17):2994-3005. doi:10.7150/jca.25876. Available from https://www.jcancer.org/v09p2994.htm

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Recent evidence indicated ubiquitin like with PHD and ring finger domains 2 (UHRF2) was involved in various human diseases, especially in cancer, however, its roles in cancer are still in dispute. Here, we found UHRF2 expression was decreased in lung cancer tissues compared with adjacent normal tissues by referring to the Oncomine Database, which was further identified by immunoblotting and quantitative real-time polymerase chain reaction assays. Secondly, we found knockdown of UHRF2 in A549 and 95-D cell lines enhanced the capability of proliferation, invasion and migration, while forced UHRF2 expression inhibited NSCLC cells proliferation,invasion and migration. Mechanistically, dot-blot and western blot assays indicated that the level of UHRF2 was positively correlated with 5-hmC level by affecting ten-eleven translocation 2 (TET2) expression. Clinically, UHRF2 downregulation is significantly correlated with a malignant phenotype, including larger tumor size and poor differentiation. Moreover, UHRF2 downregulated correlates with shorter overall survival(OS).

Conclusion: Our findings indicate that UHRF2 is a tumor suppressor in NSCLC by influence TET2 expression and serve as a potential therapeutic target in NSCLC.

Keywords: NSCLC, UHRF2, 5-hmC, demethylation