J Cancer 2019; 10(11):2541-2551. doi:10.7150/jca.29265 This issue

Research Paper

The oncogenic potential of PRR11 gene in Tongue Squamous Cell Carcinoma cells

Chunyang Wang1,2*, Liang Yu3*, Xianyue Ren1,2*, Tong Wu1,2, Xijuan Chen1,2, Yulei Huang1,2, Bin Cheng1,2✉

1. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, P.R. China
2. Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 510060, P.R. China
3. Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
*Chunyang Wang, Liang Yu and Xianyue Ren contribute equally to the work.

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Wang C, Yu L, Ren X, Wu T, Chen X, Huang Y, Cheng B. The oncogenic potential of PRR11 gene in Tongue Squamous Cell Carcinoma cells. J Cancer 2019; 10(11):2541-2551. doi:10.7150/jca.29265. Available from https://www.jcancer.org/v10p2541.htm

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Background: We previously demonstrated that Proline rich 11 (PRR11) gene is associated with the development and progression of tongue squamous cell carcinoma (TSCC), but the underlying mechanism is unknown. This study aimed to investigate the molecular mechanism underlying oncogenic potential of PRR11 in TSCC cells.

Methods: Overexpression and knockdown of PRR11 were performed by plasmid transfection into SCC15 and HSC3 human TSCC cells. Expressions of mRNA and protein were assessed by qRT-PCR and Western blot, respectively. Cell proliferation and invasion were determined by CCK-8 and Transwell assay, respectively. In vivo tumor growth and cell cycle were determined by a nude mice model of subcutaneous tumorigenesis and flow cytometry, respectively.

Results: Overexpression of PRR11 significantly enhanced TSCC cells proliferation and the invasive ability of TSCC cells, whereas PRR11 knockdown in TSCC cells exhibited a reverse trend. In addition, the in vivo subcutaneous tumorigenicity assay showed that PRR11 knockdown significantly reduced tumor size and the Ki67 (a proliferation marker)expression in the tumor tissue. Flow cytometry analysis revealed that PRR11 overexpression significantly decreased the proportion of cells in S phase, whereas PRR11 knockdown in TSCC cells exhibited a reverse trend. Furthermore, PRR11 overexpression simultaneously down-regulated two cyclin-dependent kinase inhibitors (CKIs), p21 and p27 and up-regulated CDK2 and Cyclin A2 in TSCC cells. PRR11 knockdown again exhibited reverse trends of expressions of the above proteins.Conclusion: These results suggested that PRR11 promoted cell proliferation by regulating the expressions of p21, p27, CDK2 and Cyclin A to facilitate S/G phase transition in TSCC cells.

Keywords: Proline rich 11 (PRR11), tongue squamous cell carcinoma (TSCC), oncogene, S phase arrest