J Cancer 2020; 11(1):142-152. doi:10.7150/jca.35000 This issue

Research Paper

Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45+CD19- cells but in the ALDHhigh Cells

Shupeng Song1, Yongguo Li1✉, Kaili Zhang1, Xi Zhang1, Yanxin Huang1, Mingyan Xu1, Shuangxing Li1, Xue Guan2, Tao Yang3, Zhiyu Liu4, Jie Jiang5, Yunping Luo6, Yinghua Lan1✉

1. Department of Infectious Disease, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
2. Animal experimental center, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
3. Instrument Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150000, Hei Longjiang, China.
4. Central Laboratory, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
5. Department of Pathology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
6. Department of Immunology, Institute of Basic Medical Science, Chinese Academy of Medical Science and Peking Union Medical College, Beijing,10005, China.

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Citation:
Song S, Li Y, Zhang K, Zhang X, Huang Y, Xu M, Li S, Guan X, Yang T, Liu Z, Jiang J, Luo Y, Lan Y. Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45+CD19- cells but in the ALDHhigh Cells. J Cancer 2020; 11(1):142-152. doi:10.7150/jca.35000. Available from https://www.jcancer.org/v11p0142.htm

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Abstract

Although the existence of cancer stem cells (CSCs) has been suggested in diffuse large B cell lymphoma (DLBCL), there is still no definitive marker. CD45+CD19- has been regarded as a potential marker of CSCs in mantle cell lymphoma (MCL). So, we explored the role of CD45+CD19- in DLBCL. However, both CD45+CD19- cells and CD45+CD19+ cells did not generate tumors until more than 100,000 cells were inoculated in NOD/SCID mice, even CD45+CD19+ cells generated more and larger tumors, as well as the soft agar colony formation in vitro; The aldehyde dehydrogenase (ALDH) activity was also identified in this study. Only 1,500 ALDHhigh cells were enough to generate tumors in mice while the same number of ALDH- cells were not. Moreover, both groups formed tumors when more cells were inoculated, but ALDHhigh cells formed more and larger tumors. The similar result was obtained in vitro clonogenicity experiments. OCT4, SOX2, Nanog, and ABCG2 genes did not show any difference in CD45+CD19+, CD45+CD19-, ALDHhigh and ALDH- cells. Taken together, CSCs are not enriched in the CD45+CD19- cells but in the ALDHhigh cells in DLBCL cell lines.

Keywords: CD45+CD19- , ALDH activity, CSCs markers, diffuse large B cell lymphoma