Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45+CD19- cells but in the ALDHhigh Cells

Song, Shupeng; Li, Yongguo; Zhang, Kaili; Zhang, Xi; Huang, Yanxin; Xu, Mingyan; Li, Shuangxing; Guan, Xue; Yang, Tao; Liu, Zhiyu; Jiang, Jie; Luo, Yunping; Lan, Yinghua

doi:10.7150/jca.35000

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J Cancer 2020; 11(1):142-152. doi:10.7150/jca.35000 This issue Cite

Research Paper

Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45⁺CD19^- cells but in the ALDH^high Cells

Shupeng Song¹, Yongguo Li^1✉, Kaili Zhang¹, Xi Zhang¹, Yanxin Huang¹, Mingyan Xu¹, Shuangxing Li¹, Xue Guan², Tao Yang³, Zhiyu Liu⁴, Jie Jiang⁵, Yunping Luo⁶, Yinghua Lan^1✉

1. Department of Infectious Disease, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
2. Animal experimental center, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
3. Instrument Center, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150000, Hei Longjiang, China.
4. Central Laboratory, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
5. Department of Pathology, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, Hei Longjiang, China.
6. Department of Immunology, Institute of Basic Medical Science, Chinese Academy of Medical Science and Peking Union Medical College, Beijing,10005, China.

Citation:

Song S, Li Y, Zhang K, Zhang X, Huang Y, Xu M, Li S, Guan X, Yang T, Liu Z, Jiang J, Luo Y, Lan Y. Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45⁺CD19^- cells but in the ALDH^high Cells. J Cancer 2020; 11(1):142-152. doi:10.7150/jca.35000. https://www.jcancer.org/v11p0142.htm

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Abstract

Although the existence of cancer stem cells (CSCs) has been suggested in diffuse large B cell lymphoma (DLBCL), there is still no definitive marker. CD45⁺CD19^- has been regarded as a potential marker of CSCs in mantle cell lymphoma (MCL). So, we explored the role of CD45⁺CD19^- in DLBCL. However, both CD45⁺CD19^- cells and CD45⁺CD19⁺ cells did not generate tumors until more than 100,000 cells were inoculated in NOD/SCID mice, even CD45⁺CD19⁺ cells generated more and larger tumors, as well as the soft agar colony formation in vitro; The aldehyde dehydrogenase (ALDH) activity was also identified in this study. Only 1,500 ALDH^high cells were enough to generate tumors in mice while the same number of ALDH^- cells were not. Moreover, both groups formed tumors when more cells were inoculated, but ALDH^high cells formed more and larger tumors. The similar result was obtained in vitro clonogenicity experiments. OCT4, SOX2, Nanog, and ABCG2 genes did not show any difference in CD45⁺CD19⁺, CD45⁺CD19^-, ALDH^high and ALDH^- cells. Taken together, CSCs are not enriched in the CD45⁺CD19^- cells but in the ALDH^high cells in DLBCL cell lines.

Keywords: CD45⁺CD19^- , ALDH activity, CSCs markers, diffuse large B cell lymphoma

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APA

Song, S., Li, Y., Zhang, K., Zhang, X., Huang, Y., Xu, M., Li, S., Guan, X., Yang, T., Liu, Z., Jiang, J., Luo, Y., Lan, Y. (2020). Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45⁺CD19^- cells but in the ALDH^high Cells. Journal of Cancer, 11(1), 142-152. https://doi.org/10.7150/jca.35000.

ACS

Song, S.; Li, Y.; Zhang, K.; Zhang, X.; Huang, Y.; Xu, M.; Li, S.; Guan, X.; Yang, T.; Liu, Z.; Jiang, J.; Luo, Y.; Lan, Y. Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45⁺CD19^- cells but in the ALDH^high Cells. J. Cancer 2020, 11 (1), 142-152. DOI: 10.7150/jca.35000.

NLM

CSE

Song S, Li Y, Zhang K, Zhang X, Huang Y, Xu M, Li S, Guan X, Yang T, Liu Z, Jiang J, Luo Y, Lan Y. 2020. Cancer Stem Cells of Diffuse Large B Cell Lymphoma Are Not Enriched in the CD45⁺CD19^- cells but in the ALDH^high Cells. J Cancer. 11(1):142-152.

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