J Cancer 2020; 11(1):190-198. doi:10.7150/jca.36954 This issue

Research Paper

C-MYC, BCL2 and BCL6 Translocation in B-cell Non-Hodgkin Lymphoma Cases

Dayang Sharyati Datu Abdul Salam1, Ei Ei Thit2, Siew Hoon Teoh1, Soo Yong Tan3, Suat Cheng Peh4, Shiau-Chuen Cheah1✉

1. Faculty of Medicine and Health Sciences, UCSI University, Kuala Lumpur, Malaysia.
2. Advanced Molecular Pathology Laboratory, SingHealth Tissue Repository, Singapore.
3. National University Hospital, Singapore.
4. Sunway University Hospital, Selangor, Malaysia.

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Citation:
Salam DSDA, Thit EE, Teoh SH, Tan SY, Peh SC, Cheah SC. C-MYC, BCL2 and BCL6 Translocation in B-cell Non-Hodgkin Lymphoma Cases. J Cancer 2020; 11(1):190-198. doi:10.7150/jca.36954. Available from https://www.jcancer.org/v11p0190.htm

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Abstract

C-MYC, BCL2 and BCL6 genes are the most commonly oncogenes involved in B-Cell lymphomas. Translocations of these oncogenes are associated with an aggressive clinical course. This study aims to elucidate the patterns of BCL6, BCL2 and C-MYC gene aberrations among Malaysian B-cell Non-Hodgkin Lymphoma (NHL) using fluorescence in situ hybridization (FISH). Eighty-one B-cell NHL tissue blocks were retrieved between the year 2011 to 2015 and investigated using immunohistochemistry and interphase FISH dual colour break-apart probes of BCL2, BCL6, C-MYC and IgH. A significant difference was detected between the nodal and extranodal sites in all the BCL2 (p=0.01), C-MYC (p=0.03) and IgH (p=0.006) cases except for BCL6 (p=0.2). Our study showed that BCL6 had the highest gene translocation while BCL2/BCL6 had the most mixed aberrations of gain copies and translocation, however no mixed aberrations of gain copies and translocation was found in C-MYC. None of the mixed gain copies and translocation was found in any of the germinal centre B-cell (GCB) subtype of Diffuse Large B-cell Lymphoma, however, five were found in BCL6 and IgH gene in the non-GCB subtype; while mixed gain copies and translocation cases of BCL2 gene was found in the Follicular Lymphoma cases only. The study found interesting findings of BCL2, C-MYC and IgH gene aberrations between nodal and extranodal sites. This information might benefit future study in predicting prognosis and determine effective therapeutic strategies in the multi-ethnic populations of Malaysia as well as the Asian population.

Keywords: B-cell Non-Hodgkin Lymphoma, C-MYC, BCL2, BCL6, gene abberation, FISH