J Cancer 2020; 11(2):353-363. doi:10.7150/jca.32850 This issue

Research Paper

Depletion of CDC5L inhibits bladder cancer tumorigenesis

Ziwei Zhang*, Weipu Mao*, Longsheng Wang*, Mengnan Liu, Wentao Zhang, Yuan Wu, Junfeng Zhang, Shiyu Mao, Jiang Geng, Xudong Yao

Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, P. R. China.
* Contributed equally.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Citation:
Zhang Z, Mao W, Wang L, Liu M, Zhang W, Wu Y, Zhang J, Mao S, Geng J, Yao X. Depletion of CDC5L inhibits bladder cancer tumorigenesis. J Cancer 2020; 11(2):353-363. doi:10.7150/jca.32850. Available from https://www.jcancer.org/v11p0353.htm

File import instruction

Abstract

Cell division cycle 5-like (CDC5L) protein is a cell cycle regulator of the G2/M transition and has been reported to participate in the catalytic step of pre-messenger RNA (mRNA) splicing and DNA damage repair. Recently, CDC5L was also found to act as a candidate oncogene in osteosarcoma and cervical tumours. However, the role of CDC5L expression in bladder cancer remains unclear. Here, we analysed the expression and clinical significance of CDC5L in bladder cancer tissues. The expression of CDC5L in fresh bladder cancer tissues and paraffin-embedded slices was evaluated by western blot and immunohistochemistry, respectively. We found that CDC5L was highly expressed in bladder cancer. The expression of CDC5L was significantly associated with bladder cancer pathology grade and Ki67 expression. Univariate and multivariate analyses showed that high CDC5L expression was an independent prognostic factor for the survival of bladder cancer patients. To determine whether CDC5L could regulate the proliferation of bladder cancer cells, we transfected bladder cancer cells with an interfering RNA targeting CDC5L and then investigated cell proliferation with a cell counting kit (CCK)-8, flow cytometry assays, colony formation and xenograft assay analyses. Our results indicate that knockdown of CDC5L inhibits proliferation of bladder cancer cells. In addition, reduced expression of CDC5L induced apoptosis of bladder cancer cells and inhibited their migration, invasion and EMT. These findings suggest that CDC5L might play an important role in bladder cancer and thus be a promising therapeutic target of bladder cancer.

Keywords: CDC5L, bladder cancer, tumorigenesis.