TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers

Dai, Jiaqi; Yu, Xiaqing; Han, Yali; Chai, Li; Liao, Yina; Zhong, Peng; Xie, Ruting; Sun, Xuechen; Huang, Qingqing; Wang, Jian; Yin, Zhiqiang; Zhang, Yun; Lv, Zhongwei; Jia, Chengyou

doi:10.7150/jca.47290

International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

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J Cancer 2020; 11(20):6122-6132. doi:10.7150/jca.47290 This issue Cite

Research Paper

TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers

Jiaqi Dai¹, Xiaqing Yu², Yali Han¹, Li Chai², Yina Liao¹, Peng Zhong², Ruting Xie³, Xuechen Sun³, Qingqing Huang², Jian Wang⁴, Zhiqiang Yin¹, Yun Zhang¹, Zhongwei Lv^1,2✉, Chengyou Jia^1✉

1. Shanghai Research Center for Thyroid Diseases, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, 200072, P. R. China.
2. Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, P. R. China.
3. Department of Pathology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, P. R. China.
4. Department of Nuclear Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, P. R. China.

Citation:

Dai J, Yu X, Han Y, Chai L, Liao Y, Zhong P, Xie R, Sun X, Huang Q, Wang J, Yin Z, Zhang Y, Lv Z, Jia C. TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. J Cancer 2020; 11(20):6122-6132. doi:10.7150/jca.47290. https://www.jcancer.org/v11p6122.htm

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Abstract

Background and Aim: Invasion and metastasis are critical events in papillary thyroid carcinoma (PTC) progression. Protein markers specific to this process may avoid over-treatment and urgently needed.

Methods: TMT-labeled mass spectrometry-based proteomics were carried out on PTC and invasive phenotype (iPTC) (3 pairs per group) and cross validate differentially expressed proteins (DEPs) (FC>1.5 and <0.67 and p<0.05) with GEO and TCGA datasets and the correlation genes of DEPs were also analyzed.

Results: We identified and quantified 4607 proteins identical to PTC and iPTC groups. Among which 12 DEPs in PTC and 179 DEPs in iPTCs were found. Cross-validation with GSE60542 and TCGA database revealed 10 DEPs that all significant correlated with metastasis and staging. Upregulated SLC27A6 showed negative correlation with 6 out of 9 downregulated DEPs including HGD, CA4, COL23A1, SLC26A7, FHL1 and TPO.

Conclusion: The panel of 7 genes (SLC27A6 and 6 downregulated DEPs) could have ideal prediction value to improve our understanding of invasiveness of PTC.

Keywords: papillary thyroid carcinoma, tandem-mass tag, markers, invasiveness, differentially expressed proteins

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APA

Dai, J., Yu, X., Han, Y., Chai, L., Liao, Y., Zhong, P., Xie, R., Sun, X., Huang, Q., Wang, J., Yin, Z., Zhang, Y., Lv, Z., Jia, C. (2020). TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. Journal of Cancer, 11(20), 6122-6132. https://doi.org/10.7150/jca.47290.

ACS

Dai, J.; Yu, X.; Han, Y.; Chai, L.; Liao, Y.; Zhong, P.; Xie, R.; Sun, X.; Huang, Q.; Wang, J.; Yin, Z.; Zhang, Y.; Lv, Z.; Jia, C. TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. J. Cancer 2020, 11 (20), 6122-6132. DOI: 10.7150/jca.47290.

NLM

CSE

Dai J, Yu X, Han Y, Chai L, Liao Y, Zhong P, Xie R, Sun X, Huang Q, Wang J, Yin Z, Zhang Y, Lv Z, Jia C. 2020. TMT-labeling Proteomics of Papillary Thyroid Carcinoma Reveal Invasive Biomarkers. J Cancer. 11(20):6122-6132.

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