Nitroxoline inhibits bladder cancer progression by reversing EMT process and enhancing anti-tumor immunity

Xu, Naijin; Lin, Wenfeng; Sun, Jingkai; Sadahira, Takuya; Xu, Abai; Watanabe, Masami; Guo, Kai; Araki, Motoo; Li, Gonghui; Liu, Chunxiao; Nasu, Yasutomo; Huang, Peng

doi:10.7150/jca.47025

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International Journal of Medical Sciences

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Journal of Genomics

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J Cancer 2020; 11(22):6633-6641. doi:10.7150/jca.47025 This issue Cite

Research Paper

Nitroxoline inhibits bladder cancer progression by reversing EMT process and enhancing anti-tumor immunity

Naijin Xu^1,2#, Wenfeng Lin^1#, Jingkai Sun^1,3, Takuya Sadahira¹, Abai Xu³, Masami Watanabe^1,4, Kai Guo³, Motoo Araki¹, Gonghui Li², Chunxiao Liu³, Yasutomo Nasu^1,4, Peng Huang^1,3,5✉

1. Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
2. Department of Urology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China
3. Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China
4. Center for Innovative Clinical Medicine, Okayama University Hospital
5. Okayama Medical Innovation Center, Okayama University, Okayama, Japan
^# These authors contributed to the work equally and should be regarded as co-first authors.

Citation:

Xu N, Lin W, Sun J, Sadahira T, Xu A, Watanabe M, Guo K, Araki M, Li G, Liu C, Nasu Y, Huang P. Nitroxoline inhibits bladder cancer progression by reversing EMT process and enhancing anti-tumor immunity. J Cancer 2020; 11(22):6633-6641. doi:10.7150/jca.47025. https://www.jcancer.org/v11p6633.htm

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Abstract

Nitroxoline is considered to be an effective treatment for the urinary tract infections. Recently, it has been found to be effective against several cancers. However, few studies have examined the anti-tumor activity of nitroxoline in bladder cancer. The purpose of the study was to reveal the possible mechanisms how nitroxoline inhibited bladder cancer progression. In vitro assay, we demonstrated that nitroxoline inhibited bladder cancer cell growth and migration in a concentration-related manner. Western blot analysis demonstrated that nitroxoline downregulated the expressions of epithelial mesenchymal transition (EMT)-related proteins. Furthermore, treatment with nitroxoline in the C3H/He mice bladder cancer subcutaneous model resulted in significant inhibition of tumor growth. Moreover, the percentage of myeloid‐derived suppressor cells (MDSC) in peripheral blood cells significantly decreased after treatment of nitroxoline. Taken together, our results suggested that nitroxoline may be used as a potential drug for bladder cancer.

Keywords: Nitroxoline, Bladder cancer, EMT, immunotherapy preclinical model

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APA

Xu, N., Lin, W., Sun, J., Sadahira, T., Xu, A., Watanabe, M., Guo, K., Araki, M., Li, G., Liu, C., Nasu, Y., Huang, P. (2020). Nitroxoline inhibits bladder cancer progression by reversing EMT process and enhancing anti-tumor immunity. Journal of Cancer, 11(22), 6633-6641. https://doi.org/10.7150/jca.47025.

ACS

Xu, N.; Lin, W.; Sun, J.; Sadahira, T.; Xu, A.; Watanabe, M.; Guo, K.; Araki, M.; Li, G.; Liu, C.; Nasu, Y.; Huang, P. Nitroxoline inhibits bladder cancer progression by reversing EMT process and enhancing anti-tumor immunity. J. Cancer 2020, 11 (22), 6633-6641. DOI: 10.7150/jca.47025.

NLM

CSE

Xu N, Lin W, Sun J, Sadahira T, Xu A, Watanabe M, Guo K, Araki M, Li G, Liu C, Nasu Y, Huang P. 2020. Nitroxoline inhibits bladder cancer progression by reversing EMT process and enhancing anti-tumor immunity. J Cancer. 11(22):6633-6641.

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