RNA Interference-Mediated Aurora Kinase A Gene Silencing Inhibits Human Glioma Cells Proliferation and Tumor Growth in Mice

Zhang, Ge; Ren, Guanghui; Zhao, Xin; Wang, Haibo

doi:10.7150/jca.55791

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J Cancer 2021; 12(10):3024-3032. doi:10.7150/jca.55791 This issue Cite

Research Paper

RNA Interference-Mediated Aurora Kinase A Gene Silencing Inhibits Human Glioma Cells Proliferation and Tumor Growth in Mice

Ge Zhang¹, Guanghui Ren¹, Xin Zhao¹, Haibo Wang^2✉

1. Department of Neurology, Luoyang Dong Fang Hospital, The Third Affiliated Hospital of Henan University of Science & Technology, Luoyang, Henan 471003, PR China.
2. Institute of Cardiovascular Disease, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China.

Citation:

Zhang G, Ren G, Zhao X, Wang H. RNA Interference-Mediated Aurora Kinase A Gene Silencing Inhibits Human Glioma Cells Proliferation and Tumor Growth in Mice. J Cancer 2021; 12(10):3024-3032. doi:10.7150/jca.55791. https://www.jcancer.org/v12p3024.htm

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Abstract

Objective: This study aims to explore the roles of Aurora Kinase A (Aurora A) in human glioma progression and relevant molecular mechanisms involved.

Methods: RNA interference (RNAi) technology was performed to silence the Aurora A gene in human glioma cell line U251 and U87. Western blot and real-time PCR were used to determine the protein and mRNA expression levels of Aurora A. Flow cytometry was performed to analyze the cell cycle distribution and MTT was used to examine the cell viability. Annexin V/FITC double staining and Hoechst 33258 staining were carried out to examine cell apoptosis. Xenograft tumor model was established to examine the effect of Aurora A siRNA on tumor growth in vivo.

Results: RNAi-mediated Aurora A gene silencing with specific short interfering RNA (siRNA) significantly decreased Aurora A protein and mRNA expression levels in human glioma cell line U251 and U87. Aurora A knockdown in glioma cells with siRNA strongly inhibited cell proliferation, along with the accumulation of cells in the G1, G2/M phase and decrease in S phase. Furthermore, the enhancement of cell apoptosis in vitro and the suppression of xenograft tumor growth in vivo were also observed after Aurora A silencing in U251 cell. In addition, Aurora A knockdown resulted in decreased expression of anti-apoptotic protein Bcl-2 and cell cycle protein Cyclin D1, while increased expression of pro-apoptotic factor caspase-3.

Conclusion: Aurora A can be used as a candidate targeting gene and inhibition of Aurora A is a potentially promising therapy for glioblastoma.

Keywords: RNA interference, Aurora Kinase A, Glioma, Proliferation, Apoptosis, Tumor growth.

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APA

Zhang, G., Ren, G., Zhao, X., Wang, H. (2021). RNA Interference-Mediated Aurora Kinase A Gene Silencing Inhibits Human Glioma Cells Proliferation and Tumor Growth in Mice. Journal of Cancer, 12(10), 3024-3032. https://doi.org/10.7150/jca.55791.

ACS

Zhang, G.; Ren, G.; Zhao, X.; Wang, H. RNA Interference-Mediated Aurora Kinase A Gene Silencing Inhibits Human Glioma Cells Proliferation and Tumor Growth in Mice. J. Cancer 2021, 12 (10), 3024-3032. DOI: 10.7150/jca.55791.

NLM

CSE

Zhang G, Ren G, Zhao X, Wang H. 2021. RNA Interference-Mediated Aurora Kinase A Gene Silencing Inhibits Human Glioma Cells Proliferation and Tumor Growth in Mice. J Cancer. 12(10):3024-3032.

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