ISSN: 1837-9664Journal of Cancer
Global reach, higher impact
J Cancer 2021; 12(13):3819-3826. doi:10.7150/jca.48340 This issue Cite
Research Paper
GTPBP2 positively regulates the invasion, migration and proliferation of non-small cell lung cancer
1. Department of Pathology, The Fourth Hospital Affiliated to China Medical University, Shenyang, Liaoning. 110032 People's Republic of China.
2. Department of Pathology, The First Affiliated Hospital of Anhui Medical University, Jixi street, Hefei, Anhui. 230000 People's Republic of China.
3. Department of Pathology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330000. People's Republic of China.
* These authors made equal contributions to the article.
Abstract
Lung cancer is one of the most common malignant tumors in the world, and the mortality rate ranks first among various malignant tumors. GTP-binding proteins (guanosine 5'-triphosphate-binding proteins, GTPBPs) are a type of protein with signal transduction function, have GTP hydrolase activity, and play an important role in cell signal transmission, cytoskeletal regulation, protein synthesis and other activities. GTPBP2 is one of the members of the G protein superfamily. Research on GTPBP2 is currently focused on human genetics, and its research in tumors has not been reported. First, Western blot and quantitative real-time PCR were used to analyze the expression differences of 12 cases of GTPBP2 in human NSCLC fresh cancer tissues and adjacent tissues. Then we selected 112 cases of NSCLC cancer tissues and 65 adjacent tissues for immunohistochemistry experiments to analyze the relationships between the expression of GTPBP2 and clinical pathological parameters and prognosis, we found that GTPBP2 is highly expressed in NSCLC cancer tissues, and the high expression of GTPBP2 is related to pTNM stage and lymph node metastasis. In addition, after GTPBP2 knockdown, GTPBP2 can promote the proliferation and invasion of NSCLC cell lines by up-regulating RhoC and MMP-9, and up-regulate cyclinD1, CDK4 and c-myc, and down-regulate P27 to promote the invasion of NSCLC cell lines. In addition, GTPBP2 negatively regulates Axin to promote β-catenin expression, thereby activating Wnt/β-catenin signaling, and promoting the occurrence of NSCLC.
Keywords: GTPBP2, cell proliferation, cell invasion, cell migration, non-small cell lung cancer.
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