CMTM3 Overexpression Predicts Poor Survival and Promotes Proliferation and Migration in Pancreatic Cancer

Zhou, Zixuan; Ma, Zuyi; Li, Zhenchong; Zhuang, Hongkai; Liu, Chunsheng; Gong, Yuanfeng; Huang, Shanzhou; Zhang, Chuanzhao; Hou, Baohua

doi:10.7150/jca.57082



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2021; 12(19):5797-5806. doi:10.7150/jca.57082 This issue Cite

Research Paper

CMTM3 Overexpression Predicts Poor Survival and Promotes Proliferation and Migration in Pancreatic Cancer

Zixuan Zhou, MD^1,2#, Zuyi Ma, MD^2,3#, Zhenchong Li, MD^1,2#, Hongkai Zhuang, MD^2,3, Chunsheng Liu, MD^2,3, Yuanfeng Gong, MD², Shanzhou Huang, MD, PhD^1,2,4✉, Chuanzhao Zhang, MD, PhD^1,2,4✉, Baohua Hou, MD, PhD^1,2,4✉

1. South China University of Technology School of Medicine, Guangzhou 510006,Guangdong Province, China.
2. Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China.
3. Shantou University of Medical College, Shantou 515000, China.
4. The Second School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China.
#Co-first authors with equal contributions to this work.

Citation:

Zhou Z, Ma Z, Li Z, Zhuang H, Liu C, Gong Y, Huang S, Zhang C, Hou B. CMTM3 Overexpression Predicts Poor Survival and Promotes Proliferation and Migration in Pancreatic Cancer. J Cancer 2021; 12(19):5797-5806. doi:10.7150/jca.57082. https://www.jcancer.org/v12p5797.htm

Other styles

Abstract

Background: Recent evidence has shown that CKLF-like MARVEL transmembrane domain containing 3 (CMTM3) promoted carcinogenesis and tumor progression in a variety of cancer types. The goal of our study is to investigate the association between CMTM3 and pancreatic cancer (PC).

Materials and Methods: In current study, data from public databases was used to analyze CMTM3 expression in PC. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC) were used to investigate CMTM3 expression and determine its clinical significance in PC. Then CMTM3 promoting PC aggressiveness was demonstrated in vitro experiments by cell proliferation and migration assay. Functional and pathway enrichment analyses were performed to evaluate the potential role of CMTM3 in PC.

Results: Results of qRT-PCR and IHC revealed that CMTM3 was significantly overexpressed in PC tissues. High CMTM3 expression was an independent risk factor for poor prognosis of PC patients. Overexpression of CMTM3 was associated with poor overall survival (P-value =0.031) and disease-free survival (P-value =0.0047) in the TCGA cohort. Functional and pathway enrichment analyses showed that CMTM3 were enriched in “Regulation of cell proliferation and regulation of cell differentiation, cell morphogenesis, regulation of cell differentiation, Hedgehog signaling pathway, Wnt signaling pathway, ECM-receptor interaction and pathways in cancer”. In PC cell lines, CCK8, clone formation and transwell assays showed that CMTM3 knockdown inhibited cells proliferation and migration.

Conclusion: CMTM3 was overexpressed and promotes tumor aggressiveness in PC. Our findings provided a novel therapeutic target for PC.

Keywords: CMTM3, pancreatic cancer, prognosis, cancer aggressiveness

Citation styles

APA

Zhou, Z., Ma, Z., Li, Z., Zhuang, H., Liu, C., Gong, Y., Huang, S., Zhang, C., Hou, B. (2021). CMTM3 Overexpression Predicts Poor Survival and Promotes Proliferation and Migration in Pancreatic Cancer. Journal of Cancer, 12(19), 5797-5806. https://doi.org/10.7150/jca.57082.

ACS

Zhou, Z.; Ma, Z.; Li, Z.; Zhuang, H.; Liu, C.; Gong, Y.; Huang, S.; Zhang, C.; Hou, B. CMTM3 Overexpression Predicts Poor Survival and Promotes Proliferation and Migration in Pancreatic Cancer. J. Cancer 2021, 12 (19), 5797-5806. DOI: 10.7150/jca.57082.

NLM

CSE

Zhou Z, Ma Z, Li Z, Zhuang H, Liu C, Gong Y, Huang S, Zhang C, Hou B. 2021. CMTM3 Overexpression Predicts Poor Survival and Promotes Proliferation and Migration in Pancreatic Cancer. J Cancer. 12(19):5797-5806.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.