A novel lncRNA, RPL34-AS1, promotes proliferation and angiogenesis in glioma by regulating VEGFA

Zhang, Dongzhi; Jiang, Haiping; Ye, Junyi; Gao, Ming; Wang, Xinzhuang; Lu, Enzhou; Yang, He; Wang, Lixiang; Zhao, Shiguang

doi:10.7150/jca.59337

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J Cancer 2021; 12(20):6189-6197. doi:10.7150/jca.59337 This issue Cite

Research Paper

A novel lncRNA, RPL34-AS1, promotes proliferation and angiogenesis in glioma by regulating VEGFA

Dongzhi Zhang^1,2,3,5, Haiping Jiang^1,2,3, Junyi Ye^1,2,3, Ming Gao^1,2,3, Xinzhuang Wang^1,2,3, Enzhou Lu^1,2,3, He Yang^1,2,3, Lixiang Wang^1,2,3, Shiguang Zhao^1,2,3,4✉

1. Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
2. Key Colleges and Universities Laboratory of Neurosurgery in Heilongjiang Province, Harbin, China.
3. Institute of Neuroscience, Sino-Russian Medical Research Center, Harbin Medical University, Harbin, China.
4. Shenzhen University General Hospital, Xueyuan AVE 1098, Nanshan District, 11, Shenzhen, Guangdong, P. R. China.
5. Department of Neurosurgery, The Affiliated Cancer Hospital of Harbin Medical University, Harbin, China.

Citation:

Zhang D, Jiang H, Ye J, Gao M, Wang X, Lu E, Yang H, Wang L, Zhao S. A novel lncRNA, RPL34-AS1, promotes proliferation and angiogenesis in glioma by regulating VEGFA. J Cancer 2021; 12(20):6189-6197. doi:10.7150/jca.59337. https://www.jcancer.org/v12p6189.htm

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Abstract

Purpose: Brain gliomas are the most common primary malignant tumors of the central nervous system and one of the leading causes of death in patients with intracranial tumors. The lncRNA RPL34-AS1 is significantly upregulated in glioma tissues. However, the biological function of RPL34-AS1, especially in proliferation in glioma, remains unclear.

Methods: The role of RPL34-AS1 in proliferation and angiogenesis in glioma cells was investigated using the LN229, U87, and U251 glioma cell lines. The levels of RPL34-AS1 were detected using real-time quantitative reverse transcription polymerase chain reaction. CCK-8 and colony formation assays were performed to determine the role of RPL34-AS1 in proliferation and survival, and its role in angiogenesis was assessed by an endothelial tube formation assay. Changes in protein levels were assessed by western blotting.

Results: RPL34-AS1 was upregulated in glioma tissues and was correlated with tumor grade. RPL34-AS1 expression was also higher in glioma cells than in normal astrocytes. Knockdown of RPL34-AS1 blocked glioma cell proliferation by inhibiting angiogenesis. This effect occurred through decreased ERK/AKT signaling.

Conclusions: This study suggests that RPL34-AS1 affects cell proliferation and angiogenesis in glioma and therefore may potentially serve as a valuable diagnostic and prognostic biomarker and therapeutic target in patients with glioma.

Keywords: lncRNA, RPL34-AS1, glioma, proliferation, angiogenesis, VEGFA

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APA

Zhang, D., Jiang, H., Ye, J., Gao, M., Wang, X., Lu, E., Yang, H., Wang, L., Zhao, S. (2021). A novel lncRNA, RPL34-AS1, promotes proliferation and angiogenesis in glioma by regulating VEGFA. Journal of Cancer, 12(20), 6189-6197. https://doi.org/10.7150/jca.59337.

ACS

Zhang, D.; Jiang, H.; Ye, J.; Gao, M.; Wang, X.; Lu, E.; Yang, H.; Wang, L.; Zhao, S. A novel lncRNA, RPL34-AS1, promotes proliferation and angiogenesis in glioma by regulating VEGFA. J. Cancer 2021, 12 (20), 6189-6197. DOI: 10.7150/jca.59337.

NLM

CSE

Zhang D, Jiang H, Ye J, Gao M, Wang X, Lu E, Yang H, Wang L, Zhao S. 2021. A novel lncRNA, RPL34-AS1, promotes proliferation and angiogenesis in glioma by regulating VEGFA. J Cancer. 12(20):6189-6197.

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