J Cancer 2022; 13(2):508-516. doi:10.7150/jca.65587 This issue

Research Paper

The ETNK2 gene promotes progression of papillary thyroid carcinoma through the HIPPO pathway

Danni Zheng1*, Lingli Jin1*, Buran Chen1, Yufeng Qi1, Adheesh Bhandari1,2, Jialiang Wen1, Bangyi Lin1, Xiaohua Zhang1✉, Wei Zhang1✉

1. Department of Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
2. Department of Surgery, Breast and Thyroid Unit, Primera Hospital, Kathmandu, Nepal.
* These authors contributed equally to this work.

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Citation:
Zheng D, Jin L, Chen B, Qi Y, Bhandari A, Wen J, Lin B, Zhang X, Zhang W. The ETNK2 gene promotes progression of papillary thyroid carcinoma through the HIPPO pathway. J Cancer 2022; 13(2):508-516. doi:10.7150/jca.65587. Available from https://www.jcancer.org/v13p0508.htm

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Abstract

Graphic abstract

Thyroid cancer is a disease with an extremely high incidence rate and is divided into papillary, follicular, medullary, and undifferentiated thyroid cancers. Among them, papillary carcinoma is the most common subtype. We assessed expression of ETNK2 in public databases and found that ETNK2 is upregulated in PTC. Cohort and RNA sequencing data were used to verify this discovery. To further determine the relationship between ETNK2 and papillary thyroid carcinoma, we performed an in vitro experiment. In a PTC cell line, silencing ETNK2 inhibited cell proliferation, weakened cell migration and invasion ability, promoted apoptosis, and blocked the cell cycle. In addition, western blotting suggested that ETNK2 is related to the HIPPO pathway and may activate the EMT pathway through the HIPPO pathway to promote the development of thyroid cancer. These results revealed that ETNK2 is related to the occurrence and development of papillary thyroid carcinoma, suggesting that ETNK2 may be an oncogene associated with PTC.

Keywords: ETNK2, PTC, Hippo, EMT