J Cancer 2022; 13(2):706-714. doi:10.7150/jca.65214 This issue

Research Paper

Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer

Yixi Su1,2, Qiang Huang3, Li Lu3, Hu Qu3, Dejuan Wang3, Jianguang Qiu3, Weiqian Li1, Mengmeng Lin1, Huanliang Liu1,2, Zhongyang Wang3✉, Xiangling Yang1,2✉

1. Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
2. Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.
3. Department of Urology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.

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Citation:
Su Y, Huang Q, Lu L, Qu H, Wang D, Qiu J, Li W, Lin M, Liu H, Wang Z, Yang X. Promoter Methylation-Mediated NPTX2 Silencing Promotes Tumor Growth in Human Prostate Cancer. J Cancer 2022; 13(2):706-714. doi:10.7150/jca.65214. Available from https://www.jcancer.org/v13p0706.htm

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Abstract

Graphic abstract

Neuronal pentraxin 2 (NPTX2), a secretory protein of neuronal pentraxins, was first identified in the nervous system. Several studies have shown that expression levels of NPTX2 are associated with the development of various cancers. However, whether NPTX2 is involved in prostate cancer progression is unclear. Herein, we found that NPTX2 is significantly reduced in prostate cancer tissues and cancer cell lines compared to control prostate tissues and control prostatic epithelial cell lines. Furthermore, the NPTX2 promoter is highly methylated in prostate cancer cells. Consistently, NPTX2 could be restored by treatment with the DNA methyltransferase inhibitor 5-aza-2′-deoxycytidine (decitabine, 5-AZA-dC). Overexpression of NPTX2 inhibited prostate cancer cell proliferation both in vitro and in vivo. In conclusion, our study demonstrated that NPTX2 acts as a tumor suppressor gene in prostate cancer.

Keywords: cancer targeted therapy, DNA methylation, demethylation, NPTX2, prostate cancer