J Cancer 2022; 13(3):890-905. doi:10.7150/jca.66903 This issue Cite

Research Paper

Exosomal miR-552-5p promotes tumorigenesis and disease progression via the PTEN/TOB1 axis in gastric cancer

Lingyu Zhu1, Suisui Zhang2, Songda Chen1, Huijie Wu1, Mengjie Jiang1, Aiqun Liu1✉

1. Department of Endoscopy Center, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, 530021, China
2. Department of Anesthesiology, The Second Affiliated Hospital of Chongqing Medical University, 400010, Chongqing, China.

Citation:
Zhu L, Zhang S, Chen S, Wu H, Jiang M, Liu A. Exosomal miR-552-5p promotes tumorigenesis and disease progression via the PTEN/TOB1 axis in gastric cancer. J Cancer 2022; 13(3):890-905. doi:10.7150/jca.66903. https://www.jcancer.org/v13p0890.htm
Other styles

File import instruction

Abstract

Graphic abstract

Purpose Gastric cancer (GC) is associated with rapid disease progression and poor patient prognosis, highlighting the pressing need for new biomarkers to facilitate disease management. Exosomes are released by all cells and are ubiquitous in body fluids, thus giving them great potential as diagnostic biomarkers and therapeutic targets. MicroRNAs (miRNAs) can be transported by exosomes, and are a common target for regulation in cancer.

Methods Our screen of miRNAs in the Gene Expression Omnibus and The Cancer Genome Atlas databases identified miR-552-5p as the most overexpressed miRNA in GC, and we investigated its function and mechanism of action.

Results We detected high expression of miR-552-5p in GC tissues, plasma samples and cell lines. We found that miR-552-5p binds directly to the 3′-untranslated region of PTEN, and the resulting downregulation of PTEN in turn downregulates the tumor suppressor TOB1. Furthermore, experiments in cell culture and mice showed that miR-552-5p in exosomes is internalized by recipient cells, where it enhances proliferation, migration and the epithelial-mesenchymal transition, while suppressing the caspase-3 apoptotic pathway. These effects were reversed by inhibiting miR-552-5p.

Conclusion GC-derived exosomal miR-552-5p facilitates tumorigenesis by interfering with the PTEN/TOB1 axis, providing new potential therapeutic targets.

Keywords: Exosomes, miR-552-5p, gastric cancer, PTEN, TOB1


Citation styles

APA
Zhu, L., Zhang, S., Chen, S., Wu, H., Jiang, M., Liu, A. (2022). Exosomal miR-552-5p promotes tumorigenesis and disease progression via the PTEN/TOB1 axis in gastric cancer. Journal of Cancer, 13(3), 890-905. https://doi.org/10.7150/jca.66903.

ACS
Zhu, L.; Zhang, S.; Chen, S.; Wu, H.; Jiang, M.; Liu, A. Exosomal miR-552-5p promotes tumorigenesis and disease progression via the PTEN/TOB1 axis in gastric cancer. J. Cancer 2022, 13 (3), 890-905. DOI: 10.7150/jca.66903.

NLM
Zhu L, Zhang S, Chen S, Wu H, Jiang M, Liu A. Exosomal miR-552-5p promotes tumorigenesis and disease progression via the PTEN/TOB1 axis in gastric cancer. J Cancer 2022; 13(3):890-905. doi:10.7150/jca.66903. https://www.jcancer.org/v13p0890.htm

CSE
Zhu L, Zhang S, Chen S, Wu H, Jiang M, Liu A. 2022. Exosomal miR-552-5p promotes tumorigenesis and disease progression via the PTEN/TOB1 axis in gastric cancer. J Cancer. 13(3):890-905.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.