J Cancer 2022; 13(5):1398-1409. doi:10.7150/jca.58053 This issue
1. Department of Interventional Oncology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
2. Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China.
3. Department of Urology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
4. Department of clinical medicine, Medical School of Nantong University.
5. Department of Hepatobiliary Surgery, Tenth People's Hospital of Tongji University, Shanghai, China, 200072.
6. Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Naval, Medical University, Shanghai, China, 200438.
7. Department of Urology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, P.R. China.
8. Department of Transplantation, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
#Theses authors contributed equally to this work.
Clear cell renal cell carcinoma (ccRCC) has become a common malignant cancer with increasing incidence rate and high recurrence risk in genitourinary oncology around the world. Recently, miRNAs were identified to affect pathogenesis, development, molecular functions, and prognosis of ccRCC. In this study, microRNA-184-5p (miR-184-5p) was identified from three independent ccRCC cohorts and was determined as a significantly distinct prognostic biomarker. Relative miR-184-5p expression was found in A-498 and 786-O ccRCC cells compared with HK-2 cells. After ccRCC cells were transfected with miR-184-5p mimics or inhibitor, biological abilities of miR-184-5p in tumor cell proliferation, cycle, apoptosis and invasion were determined. Additionally, we confirmed the direct relationship between miR-184-5p and NUS1 dehydrodolichyl diphosphate synthase subunit (NUS1) by using the Luciferase reporter and rescue assays. These results indicated that the expression level of miR-184-5p in human ccRCC cells and tissues was reduced, and the up-regulation of miR-184-5p regulated A-498 and 786-O cell proliferation, invasion and apoptosis by directly targeting NUS1. These findings may provide new theoretical targets for treatment strategies and drug development of ccRCC.
Keywords: Clear cell renal cell carcinoma, microRNA, NUS1, invasion, proliferation, biomarker