J Cancer 2022; 13(9):2970-2981. doi:10.7150/jca.71706 This issue

Research Paper

Blood circulating exosomes carrying microRNA-423-5p regulates cell progression in prostate cancer via targeting FRMD3

Yongbao Wei1,2§, Zhensheng Chen, Ruochen Zhang1,2§, Bangkui Wu4, Le Lin1,2, Qingguo Zhu1,2, Liefu Ye1,2, Tao Li1,2✉, Feng Li1,5✉

1. Shengli Clinical Medical College of Fujian Medical University, Fuzhou, China
2. Department of Urology, Fujian Provincial Hospital, Fuzhou, China
3. Department of Urology, Fuding Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuding, Fujian Province, China
4. Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China
5. Department of Pathology, Fujian Provincial Hospital, Fuzhou, China
§Yongbao Wei, Zhensheng Chen, and Ruochen Zhang contributed equally to this work.

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Citation:
Wei Y, Chen Z, Zhang R, Wu B, Lin L, Zhu Q, Ye L, Li T, Li F. Blood circulating exosomes carrying microRNA-423-5p regulates cell progression in prostate cancer via targeting FRMD3. J Cancer 2022; 13(9):2970-2981. doi:10.7150/jca.71706. Available from https://www.jcancer.org/v13p2970.htm

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Abstract

Graphic abstract

This study explored the role of circulating exosomal microRNA-423-5p in the progression of PCa and its molecular mechanism. First, based on the microarray analysis, microRNA-423-5p was at a high expression level in PCa peripheral blood samples. It was demonstrated that microRNA-423-5p expression in serum exosomes of PCa patients was notably higher than that in healthy people as revealed by qRT-PCR. Further studies indicated that overexpressing microRNA-423-5p promoted cell progression of PCa. Microarray analysis and luciferase gene reporter assay illustrated that FRMD3 was targeted by microRNA-423-5p, and its expression was down-regulated by microRNA-423-5p. While FEMD3 knockdown would reverse the repressive effect of silencing microRNA-423-5p on PCa cell functions. In addition, it was exhibited that exosomes carrying microRNA-423-5p could internalize into PCa cells by labeling and tracing exosomes. Cell function assays and animal experiments manifested those exosomes carrying microRNA-423-5p could enhance PCa cell proliferation, migration, and invasion in vivo. In conclusion, this study indicated that blood circulating exosomal microRNA-423-5p played important roles in PCa cell functions, and illustrated the molecular mechanism of microRNA-423-5p as an oncogene in PCa.

Keywords: microRNA-423-5p, FRMD3, prostate cancer, exosome, proliferation