J Cancer 2022; 13(14):3533-3538. doi:10.7150/jca.75510 This issue Cite

Research Paper

Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines

Maria E Cuevas, Chance P Winters, Maria C Todd

Biology Department, Southwestern University, Georgetown, TX 78626.

Citation:
Cuevas ME, Winters CP, Todd MC. Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines. J Cancer 2022; 13(14):3533-3538. doi:10.7150/jca.75510. https://www.jcancer.org/v13p3533.htm
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Abstract

Graphic abstract

Deregulation of tight junction (TJ) proteins and the associated disruption of TJ function has been demonstrated to play a role in the development of endometrial cancer. In the current study, we have shown overexpression of claudin-3 and -4 mRNA (by RT-PCR) and protein (by immunoblotting) in a panel of 9 human endometrial cancer cell lines. To further expand our understanding of the complex role of TJ deregulation in endometrial cancer, we also investigated the expression of 84 TJ and TJ-associated genes (encoding the array of proteins that function within the TJ network from the membrane to nuclear signaling pathways) by microarray analysis. Consistent with the claudin-3 and -4 RT-PCR and immunoblot findings described above, we observed overexpression of the claudin-3 and -4 genes by microarray analysis. Further, we observed overexpression of an additional three genes in 8 of the 9 endometrial cancer cell lines: OCLN (occludin), F11R (JAM-A) and TJP3 (ZO-3). OCLN and F11R encode integral membrane proteins whereas TJP3 encodes a cytosolic scaffolding protein that indirectly links membrane TJ proteins to the actin cytoskeleton and cell signaling pathways. Our data suggest that the structural disruption of TJs coupled with the downstream deregulation of signaling pathways involved in cellular proliferation and migration may contribute to the development of endometrial cancer.

Keywords: endometrial cancer, claudin-3, claudin-4, F11R, TPJ3, microarray


Citation styles

APA
Cuevas, M.E., Winters, C.P., Todd, M.C. (2022). Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines. Journal of Cancer, 13(14), 3533-3538. https://doi.org/10.7150/jca.75510.

ACS
Cuevas, M.E.; Winters, C.P.; Todd, M.C. Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines. J. Cancer 2022, 13 (14), 3533-3538. DOI: 10.7150/jca.75510.

NLM
Cuevas ME, Winters CP, Todd MC. Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines. J Cancer 2022; 13(14):3533-3538. doi:10.7150/jca.75510. https://www.jcancer.org/v13p3533.htm

CSE
Cuevas ME, Winters CP, Todd MC. 2022. Microarray Analysis Reveals Overexpression of both Integral Membrane and Cytosolic Tight Junction Genes in Endometrial Cancer Cell Lines. J Cancer. 13(14):3533-3538.

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