Enhanced Therapeutic Effects of an Antitumor Agent on Subcutaneous Tumors in Mice by Photomechanical Wave-based Transvascular Drug Delivery

Tsunoi, Yasuyuki; Tsuda, Hitoshi; Kawauchi, Satoko; Araki, Koji; Sato, Shunichi

doi:10.7150/jca.84066

International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

open access Global reach, higher impact

Full Text | PDF

J Cancer 2023; 14(10):1773-1780. doi:10.7150/jca.84066 This issue Cite

Research Paper

Enhanced Therapeutic Effects of an Antitumor Agent on Subcutaneous Tumors in Mice by Photomechanical Wave-based Transvascular Drug Delivery

Yasuyuki Tsunoi^1✉, Hitoshi Tsuda², Satoko Kawauchi¹, Koji Araki³, Shunichi Sato¹

1. Division of Bioinformation and Therapeutic Systems, National Defense Medical College Research Institute, Japan.
2. Department of Basic Pathology, National Defense Medical College, Japan.
3. Department of Otolaryngology-Head and Neck Surgery, National Defense Medical College, Japan.

Citation:

Tsunoi Y, Tsuda H, Kawauchi S, Araki K, Sato S. Enhanced Therapeutic Effects of an Antitumor Agent on Subcutaneous Tumors in Mice by Photomechanical Wave-based Transvascular Drug Delivery. J Cancer 2023; 14(10):1773-1780. doi:10.7150/jca.84066. https://www.jcancer.org/v14p1773.htm

Other styles

Abstract

Purpose: We previously developed a site-selective transvascular drug delivery system based on nanosecond pulsed laser-induced photomechanical waves (PMWs). In this study, we applied this method to the delivery of cisplatin (cis-diamminedichloroplatinum, CDDP) to a subcutaneous tumor in a mouse and examined its antitumor effects.

Methods: A mouse tumor model with subcutaneous inoculation of human head and neck cancer cells (FaDu cells) was used. The mice were divided into four groups: control without any treatment (control), CDDP application only (CDDP only), PMW application only (PMW only) and combined application of PMWs and CDDP (PMW+CDDP). A PMW was generated by irradiating a laser target, which was placed on the skin over the tumor, with a ruby laser pulse (fluence, 1.6 J/cm²). A CDDP solution was intraperitoneally injected into the mice (2.5 mg/kg).

Results: Until 7 days posttreatment, the tumor volume in the control group monotonically increased, while the tumor volumes in the CDDP-only group and PMW-only group did not change greatly and that in the PMW+CDDP group slightly decreased. Afterward, the tumors started to regrow in all treatment groups, but the tumor growth rate was considerably low in the PMW+CDDP group. There was a significant difference in the time courses of tumor volume between the PMW+CDDP group and the control group for up to 14 days posttreatment. The ratio of the Ki-67-positive (proliferative) areas to the whole tumor regions in the PMW+CDDP group was significantly smaller than that in the control group at 7 days posttreatment. These results are attributable to the synergistic effects of enhanced extravasation of CDDP and mechanical tumoricidal effect by PMWs.

Conclusion: The combined application of CDDP and PMWs significantly improved the antitumor effects on mouse subcutaneous tumors.

Keywords: photomechanical wave, laser-induced stress wave, transvascular drug delivery, drug delivery system, cisplatin, chemotherapy

Citation styles

APA

Tsunoi, Y., Tsuda, H., Kawauchi, S., Araki, K., Sato, S. (2023). Enhanced Therapeutic Effects of an Antitumor Agent on Subcutaneous Tumors in Mice by Photomechanical Wave-based Transvascular Drug Delivery. Journal of Cancer, 14(10), 1773-1780. https://doi.org/10.7150/jca.84066.

ACS

Tsunoi, Y.; Tsuda, H.; Kawauchi, S.; Araki, K.; Sato, S. Enhanced Therapeutic Effects of an Antitumor Agent on Subcutaneous Tumors in Mice by Photomechanical Wave-based Transvascular Drug Delivery. J. Cancer 2023, 14 (10), 1773-1780. DOI: 10.7150/jca.84066.

NLM

CSE

Tsunoi Y, Tsuda H, Kawauchi S, Araki K, Sato S. 2023. Enhanced Therapeutic Effects of an Antitumor Agent on Subcutaneous Tumors in Mice by Photomechanical Wave-based Transvascular Drug Delivery. J Cancer. 14(10):1773-1780.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.