miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin

Zhuang, Jing; Fan, Jianjun; Zhu, Lihuan; Zhao, Lilan; Huang, Yangyun; Pan, Xiaojie; Guo, Tianxing

doi:10.7150/jca.83221

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J Cancer 2023; 14(11):2015-2022. doi:10.7150/jca.83221 This issue Cite

Research Paper

miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin

Jing Zhuang^2,#, Jianjun Fan^1,#, Lihuan Zhu¹, Lilan Zhao¹, Yangyun Huang¹, Xiaojie Pan¹, Tianxing Guo^1✉

1. Department of Thoracic Surgery, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, No. 134 East Street, Fuzhou 350001, China.
2. Department of Plastic and Burn, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, No. 134 East Street, Fuzhou 350001, China.
#These authors contributed equally to this research.

Citation:

Zhuang J, Fan J, Zhu L, Zhao L, Huang Y, Pan X, Guo T. miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin. J Cancer 2023; 14(11):2015-2022. doi:10.7150/jca.83221. https://www.jcancer.org/v14p2015.htm

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Abstract

Background: Non-small cell lung cancer (NSCLC) is a common malignant tumor, and it is characterized by high mortality. MicroRNA-452-5p (miR-452-5p) and Moesin (MSN) have been proved to be related with regulation of tumors. If miR-452-5p could regulate NSCLC through targeting MSN remain unclear.

Methods: TargetScan data and GEPIA databases were used to predict binding site and analyze gene expression, respectively. EdU staining, wound healing, and Transwell assays were performed to measure cell proliferation, migration, and invasion, respectively.

Results: The binding site between miR-452-5p and MSN was predicted and validated. Overexpression of miR-452-5p cell lines were constructed, and miR-452-5p mimics markedly inhibited the migration, invasion, and proliferation ability of both H322 and A549 cells, but these effects of miR-452-5p were reversed by pcDNA-MSN. pcDNA-MSN significantly reversed the influence of miR-452-5p mimics on the EMT related proteins expression in H322 and A549 cell lines by decreasing E-cadherin and increasing N-cadherin. Significant higher expression of MSN in lung adenocarcinoma and lung squamous cell carcinoma was observed through GEPIA and TCGA data base analysis. Higher expression of MSN is positively correlated with advanced lung cancer and suggests poor prognosis.

Conclusions: We demonstrated that miR-452-5p modulated the cell proliferation, migration, invasion, and EMT process of H322 and A549 cell lines through targeting MSN. This research might provide a novel prevention and treatment target for NSCLC.

Keywords: miR-452-5p, NSCLC, MAPK, ERK

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APA

Zhuang, J., Fan, J., Zhu, L., Zhao, L., Huang, Y., Pan, X., Guo, T. (2023). miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin. Journal of Cancer, 14(11), 2015-2022. https://doi.org/10.7150/jca.83221.

ACS

Zhuang, J.; Fan, J.; Zhu, L.; Zhao, L.; Huang, Y.; Pan, X.; Guo, T. miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin. J. Cancer 2023, 14 (11), 2015-2022. DOI: 10.7150/jca.83221.

NLM

CSE

Zhuang J, Fan J, Zhu L, Zhao L, Huang Y, Pan X, Guo T. 2023. miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin. J Cancer. 14(11):2015-2022.

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