Full Text | PDF

J Cancer 2024; 15(5):1328-1341. doi:10.7150/jca.90298 This issue Cite

Research Paper

The significance of HAUS1 and its relationship with immune microenvironment in hepatocellular carcinoma

Lei Tang^1,2,3#, Zhonghuo Chen^6#, Chao Wei^1,2,3#, Hao Liu^1,2, Ben Wang⁵, Taozhi Yu^1,2,3, Xiaofei Tao^1,2,3, Jiale Yang^1,2,3, Jiafu Guan^1,2, Jianwei Yi^1,2, Hengchang Zhu^1,2, Chen Li^1,2, Peng Tang^1,2, Kai Wang^1,2,3,4,7✉

1. Hepatobiliary and Pancreatic Surgery Division, Department of General Surgery, the 2nd affiliated hospital, Jiangxi Medical College, Nanchang University, Nanchang 330038, China.
2. Jiangxi Province Engineering Research Center of Hepatobiliary Disease, the 2nd affiliated hospital, Jiangxi Medical College, Nanchang University, Nanchang 330008, China.
3. Jiangxi Province Key Laboratory of Molecular Medicine, the 2nd affiliated hospital, Jiangxi Medical College, Nanchang University, Nanchang 330008, China.
4. Jiangxi Provincial Clinical Research Center for General Surgery Disease, the 2nd affiliated hospital, Jiangxi Medical College, Nanchang University, Nanchang 330008, China.
5. Department of General Surgery, No. 215 Hospital of Shanxi Nuclear Industry, Xianyang 712000, China.
6. Department of Emergency, Jiangxi Province Hospital of Integrated Chinese and Western Medicine, Nanchang 330009, China.
7. Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, Ministry of Education, the 2nd affiliated hospital, Jiangxi Medical College, Nanchang University, Nanchang 330031, China.
# Equal contribution.

✉ Corresponding author: Kai Wang; Phone number: +86-13767104812; FAX number: 00+86+036+079187357270; Email: ndefy07021edu.cn.

Abstract

Background: HAUS Augmin-like complex subunit 1(HAUS1), as a controlling gene, which affected the production of spindle was firstly discovered in Drosophila cells. Although HAUS1 has been intensively studied, but its significance and relationship with the immune microenvironment in Hepatocellular carcinoma (HCC) remain unclear.

Materials and Methods: All data of HCC in this paper were obtained from The Cancer Genome Atlas(TCGA), Genotype-Tissue Expression (GTEx), Gene Expression Omnibus (GEO) and the Human Protein Atlas(HPA) database. The role and potential value of HAUS1 in the tumorigenesis and development of HCC were studied by applying plenty of bioinformatics analysis methods. Knocked down the expression of HAUS1 through siRNA and further investigated the function of HAUS1 in HCC

Results: HAUS1 was highly expressed in HCC, which led to a poor prognosis. ROC curve analysis showed that HAUS1 had a excellent diagnostic value. It was also associated with clinical stage, pathological grade and AFP of HCC. Univariate and multivariate COX regression analysis showed that HAUS1 was an independent prognostic factor for HCC patients. HAUS1 was associated with immune cells infiltrate and immune checkpoints in HCC, and it could generate significative therapeutic results when combined with anti-CTLA4 and anti-CD274 treatment. In vitro experiments, HAUS1 was found to promote the proliferation, invasion and metastasis, participated in cell cycle regulation and inhibited apoptosis of HCC.

Conclusion: These results suggested that HAUS1 might serve as a potential therapeutic target, as well as a diagnostic, prognostic, and survival biomarker for HCC.

Keywords: HAUS1, HCC, Immune microenvironment, Prognosis, Biomarker

Citation styles

©2024 Ivyspring International Publisher. Terms of use