MiR-383-5p inhibits the proliferation and migration of lung adenocarcinoma cells by targeting SHMT2

Bi, Xianxia; Wang, Luwei; Li, Hua; Ma, Ying; Guo, Ruoyu; Yue, Jicheng; Kong, Lijun; Gong, Xiangqian; Jiao, Fei; Chinn, Eugene; Hu, Jinxia

doi:10.7150/jca.89733

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J Cancer 2024; 15(9):2746-2758. doi:10.7150/jca.89733 This issue Cite

Research Paper

MiR-383-5p inhibits the proliferation and migration of lung adenocarcinoma cells by targeting SHMT2

Xianxia Bi^1*, Luwei Wang^1*, Hua Li², Ying Ma³, Ruoyu Guo³, Jicheng Yue¹, Lijun Kong³, Xiangqian Gong⁴, Fei Jiao³, Eugene Chinn^1✉, Jinxia Hu^3✉

1. Peninsula Cancer Research Center of Binzhou Medical University, YanTai, Shandong 264003, P.R. China.
2. Yantai Environmental Sanitation Management Center, YanTai, Shandong 264000, P.R. China.
3. Department of Biochemistry and Molecular Biology, Binzhou Medical University, YanTai, Shandong 264003, P.R. China.
4. Department of Gastrointestinal Surgery, Yuhuangding Hospital, YanTai, Shandong 265499, P.R. China.
* These authors share first authorship.
^# These authors share corresponding authorship.

Citation:

Bi X, Wang L, Li H, Ma Y, Guo R, Yue J, Kong L, Gong X, Jiao F, Chinn E, Hu J. MiR-383-5p inhibits the proliferation and migration of lung adenocarcinoma cells by targeting SHMT2. J Cancer 2024; 15(9):2746-2758. doi:10.7150/jca.89733. https://www.jcancer.org/v15p2746.htm

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Abstract

Purpose: To explore the effects of miR-383-5p and serine hydroxymethyltransferase 2 (SHMT2) on the proliferation and migration of lung adenocarcinoma cells.

Methods: SHMT2 expression in lung adenocarcinoma and normal tissues was investigated using The Cancer Genome Atlas database. Immunohistochemical analysis was performed to confirm SHMT2 expression in lung adenocarcinoma and adjacent normal lung tissues. Bioinformatics analysis and luciferase reporter assays were used to analyze the relationship between miR-383-5p and SHMT2 expression. The protein expression levels of SHMT2, vimentin, N-cadherin, E-cadherin, Bcl-2, and cyclinD1 were analyzed using western blotting. The reverse transcription-quantitative polymerase chain reaction was used to detect SHMT2 knockdown efficiency, miR-383-5p overexpression, and inhibition efficiency. The proliferative ability of cells was detected using the Cell Counting Kit-8 assay. The Transwell assay was used to detect the migration ability of cells.

Results: SHMT2 expression was significantly increased in patients with lung adenocarcinoma compared to that in control patients; the higher the SHMT2 expression the worse the outcomes were in patients with lung adenocarcinoma. SHMT2 knockdown inhibited the proliferation, migration, and epithelial-mesenchymal transition of lung adenocarcinoma A549 and H1299 cells. MiR-383-5p directly targeted and downregulated SHMT2 in A549 and H1299 cells. The effects of miRNA-383-5p on the proliferation and migration of these cells differed from those of SHMT2. Exogenous overexpression of SHMT2 reversed the miR-383-5p-induced proliferation and migration inhibition in A549 and H1299 cells.

Conclusion: MiR-383-5p inhibits the proliferation and migration of lung adenocarcinoma cells by targeting and downregulating SHMT2.

Keywords: miR-383-5p, SHMT2, lung adenocarcinoma, proliferation, migration

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APA

Bi, X., Wang, L., Li, H., Ma, Y., Guo, R., Yue, J., Kong, L., Gong, X., Jiao, F., Chinn, E., Hu, J. (2024). MiR-383-5p inhibits the proliferation and migration of lung adenocarcinoma cells by targeting SHMT2. Journal of Cancer, 15(9), 2746-2758. https://doi.org/10.7150/jca.89733.

ACS

Bi, X.; Wang, L.; Li, H.; Ma, Y.; Guo, R.; Yue, J.; Kong, L.; Gong, X.; Jiao, F.; Chinn, E.; Hu, J. MiR-383-5p inhibits the proliferation and migration of lung adenocarcinoma cells by targeting SHMT2. J. Cancer 2024, 15 (9), 2746-2758. DOI: 10.7150/jca.89733.

NLM

CSE

Bi X, Wang L, Li H, Ma Y, Guo R, Yue J, Kong L, Gong X, Jiao F, Chinn E, Hu J. 2024. MiR-383-5p inhibits the proliferation and migration of lung adenocarcinoma cells by targeting SHMT2. J Cancer. 15(9):2746-2758.

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