A-to-I edited miR-154-p13-5p inhibited cell proliferation and migration and induced apoptosis by targeting LIX1L in the bladder cancer

Hu, Zhengxiang; Liu, Chunhui; Mei, Zujun; Wang, Xinlei; Ma, Yuyang; Liu, Xing; Xu, Hao; Fang, Gaochuan; Liu, Xinyu; Li, Rui; Wang, Jie; Shi, Zhenduo; Han, Conghui

doi:10.7150/jca.93388



International Journal of Biological Sciences

International Journal of Medical Sciences

Theranostics

Journal of Genomics

Nanotheranostics

Global reach, higher impact

Full Text | PDF

J Cancer 2024; 15(12):3708-3723. doi:10.7150/jca.93388 This issue Cite

Research Paper

A-to-I edited miR-154-p13-5p inhibited cell proliferation and migration and induced apoptosis by targeting LIX1L in the bladder cancer

Zhengxiang Hu^1,2#, Chunhui Liu^3#, Zujun Mei^4#, Xinlei Wang^3#, Yuyang Ma⁵, Xing Liu³, Hao Xu⁵, Gaochuan Fang⁶, Xinyu Liu³, Rui Li⁷, Jie Wang⁷, Zhenduo Shi^2✉, Conghui Han^2✉

1. Postgraduate Training Base of Jinzhou Medical University in The Central Hospital of Xuzhou, Jinzhou, Liaoning 121013, China.
2. Department of Urology, Xuzhou Central Hospital, Xuzhou, Jiangsu 221006, China.
3. Xuzhou Clinical College of Xuzhou Medical University, Xuzhou 221004, China.
4. Department of Emergency, Jingzhou Central Hospital, Jingzhou, Hubei 434000, China.
5. Graduate School of Bengbu Medical College, Bengbu, Anhui 233060, China.
6. School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
7. Central Laboratory, Xuzhou Central Hospital, Jiangsu 221006, China.
#These authors have contributed equally to this work.

Citation:

Hu Z, Liu C, Mei Z, Wang X, Ma Y, Liu X, Xu H, Fang G, Liu X, Li R, Wang J, Shi Z, Han C. A-to-I edited miR-154-p13-5p inhibited cell proliferation and migration and induced apoptosis by targeting LIX1L in the bladder cancer. J Cancer 2024; 15(12):3708-3723. doi:10.7150/jca.93388. https://www.jcancer.org/v15p3708.htm

Other styles

Abstract

With the advancement of RNA sequencing technology, there has been a drive to uncover and elucidate the pivotal role of A-to-I RNA editing events in tumorigenesis. However, A-to-I miRNA editing events have been clearly identified in bladder cancer, the molecular mechanisms underlying their role in bladder cancer remain unclear. In our investigation, we observed a notable under-expression of edited miR-154-p13-5p in bladder cancer (BC) tissues, in contrast to normal counterparts. Remarkably, heightened expression levels of edited miR-154-p13-5p correlated with improved survival outcomes. To assess the impact of modified miR-154-p13-5p, we conducted a string of cell phenotype assays through transfection of the corresponding miRNAs or siRNAs. The results unequivocally demonstrate that edited miR-154-p13-5p exerts a substantial inhibitory influence on proliferation, migration, and induces apoptosis by specifically targeting LIX1L in bladder cancer. Moreover, we observed that the editing of miR-154-p13-5p or LIX1L-siRNAs inhibits the expression of LIX1L, thereby suppressing EMT-related proteins and cell cycle protein CDK2. Simultaneously, an upregulation in the expression levels of Caspase-3 and Cleaved Caspase-3 were also detected. Our research findings suggest that the upregulation of edited miR-154-p13-5p could potentially enhance the prognosis of bladder cancer, thereby presenting molecular biology-based therapeutic strategies.

Keywords: bladder cancer, miRNA editing, miR-154-p13-5p, LIX1L

Citation styles

APA

Hu, Z., Liu, C., Mei, Z., Wang, X., Ma, Y., Liu, X., Xu, H., Fang, G., Liu, X., Li, R., Wang, J., Shi, Z., Han, C. (2024). A-to-I edited miR-154-p13-5p inhibited cell proliferation and migration and induced apoptosis by targeting LIX1L in the bladder cancer. Journal of Cancer, 15(12), 3708-3723. https://doi.org/10.7150/jca.93388.

ACS

Hu, Z.; Liu, C.; Mei, Z.; Wang, X.; Ma, Y.; Liu, X.; Xu, H.; Fang, G.; Liu, X.; Li, R.; Wang, J.; Shi, Z.; Han, C. A-to-I edited miR-154-p13-5p inhibited cell proliferation and migration and induced apoptosis by targeting LIX1L in the bladder cancer. J. Cancer 2024, 15 (12), 3708-3723. DOI: 10.7150/jca.93388.

NLM

CSE

Hu Z, Liu C, Mei Z, Wang X, Ma Y, Liu X, Xu H, Fang G, Liu X, Li R, Wang J, Shi Z, Han C. 2024. A-to-I edited miR-154-p13-5p inhibited cell proliferation and migration and induced apoptosis by targeting LIX1L in the bladder cancer. J Cancer. 15(12):3708-3723.

This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.