J Cancer 2014; 5(7):548-558. doi:10.7150/jca.9315 This issue

Research Paper

Metastatic Hepatocarcinoma He/De Tumor Model in Rat

Gyorgy Trencsenyi1, Terez Marian1, Fruzsina Bako1, Miklos Emri1, Gabor Nagy2, Pal Kertai3, Gaspar Banfalvi2✉

1. Department of Nuclear Medicine, University of Debrecen;
2. Department of Microbial Biotechnology and Cell Biology, University of Debrecen;
3. Department of Preventive Medicine, University of Debrecen.

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Trencsenyi G, Marian T, Bako F, Emri M, Nagy G, Kertai P, Banfalvi G. Metastatic Hepatocarcinoma He/De Tumor Model in Rat. J Cancer 2014; 5(7):548-558. doi:10.7150/jca.9315. Available from https://www.jcancer.org/v05p0548.htm

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The aim of this study is to select among potential tumor models that could be suitable to follow the metastatic spead of tumor cells. 18FDG-PET tumor diagnostic test has been adapted to investigate tumor growth in vivo in local and metastatic rat models. Materials and Methods. The expression of glucose transporters was traced by immunohistological analysis, followed by the uptake of 18FDG and visualized by MiniPET scanner. After s.c. administration of hepatocarcinoma (He/De) cells intensive local tumor growth and 18FDG uptake were measured. Results: Whole body 18FDG-PET imaging supported by histological analysis have shown that subcutaneously growing tumors did not project metastases to other sites from the injected area. To avoid local tumor formation i.v. injection was chosen, but did not improve the safety of tumor cell administration. Tumor formation after i.v. injection took a longer time than after s.c. administration. Tumors upon i.v. generation were smaller and detectable in liver and lung, but not in other organs or tissues. iii) Subrenally implanted He/De cells spread from the retroperitoneal primary tumor of the kidney to thoracal paratymic lymph nodes (PTNs). The spread from primary site to metastatic tumors in PTNs was confirmed by post mortem surgery and histological examinations. Conclusion: Among the three methods applied: a) Local s.c. administration of tumor cells generated local tumors unsuitable to study metastasis. b) Intravenous administration causing unpredicatable location of tumor formation is not regarded a reliable metastatic tumor model. c) Subreanal implantation model proved to be a suitable model to follow the metastatic process in rats.

Keywords: transplantation, metastatic models, tumor diagnostics, subrenal capsule model, miniPET, immunohistochemistry.