ISSN: 1837-9664Journal of Cancer
Global reach, higher impact
J Cancer 2015; 6(4):351-359. doi:10.7150/jca.11093 This issue Cite
Research Paper
Expression and Function of HAX-1 in Human Cutaneous Squamous Cell Carcinoma
1. Department of Pathological Anatomy, Medical School of Nantong University, Nantong 226001, Jiangsu, China;
2. Small RNA Technology and Application Institute, Nantong University, E&T Development Area, Nantong 226016, Jiangsu, China;
3. Medical School of Nantong University, Nantong 226001, Jiangsu, China;
4. Department of Oncology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China.
*The first two authors have contributed equally to this work.
Abstract
HAX-1 is an anti-apoptotic factor and overexpressed in many types of cancers. However, the functional role of HAX-1 in human cutaneous squamous cell carcinoma (cSCC) remains unclear. Our aim was to investigate the expression of HAX-1 in cSCC and its relationship with the development of cSCC. HAX-1 expression in cSCC tissues and in-vitro cell models were evaluated by real-time quantitative PCR (RT-qPCR), Western blot and immunohistochemistry. And the RNAi strategy was used to observe the relationship of HAX-1 and cSCC in A431 cells. The mRNA and protein level of HAX-1 were significantly higher in cSCC compared with normal tissue. There were significant differences in thickness (P=0.014), differentiation (P=0.027) and TNM stages (P=0.007). After knockdown the expression of HAX-1 by siRNA, the proliferation and the motility of A431 cell was inhibited obviously, and the apoptosis of A431 cells were induced too. HAX-1 may be a risk factor for patients with cSCC. As a potential tumor promoter in cSCC, HAX-1 may be a novel potential therapeutic target for cSCC treatment and deserves further investigation.
Keywords: HAX-1, Cutaneous squamous cell carcinoma (cSCC), RNA interference (RNAi), A431.
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