J Cancer 2015; 6(12):1346-1351. doi:10.7150/jca.13361 This issue Cite

Short Research Communication

Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene

Heegyum Moon1, Xuexiu Zheng1, Tiing Jen Loh, Ha Na Jang, Yongchao Liu, Da-Woon Jung, Darren R Williams, Haihong Shen

School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
1These authors contributed equally to this manuscript.

Citation:
Moon H, Zheng X, Loh TJ, Jang HN, Liu Y, Jung DW, Williams DR, Shen H. Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene. J Cancer 2015; 6(12):1346-1351. doi:10.7150/jca.13361. https://www.jcancer.org/v06p1346.htm
Other styles

File import instruction

Abstract

RON receptor tyrosine kinase is a proto-oncogene that induces cell migration and matrix invasion. RONΔ160 protein, which is produced by exclusion of exon 5 and 6, promotes cell migration, matrix invasion and protection from apoptosis. Alternative splicing regulation of exon 5 and 6 is not well understood. In this manuscript, we identified several new RNA regulatory elements for alternative splicing of Ron proto-oncogene. Firstly, we demonstrated that RNA sequences from EcoRI cleavage sites regulate alternative splicing of Ron exon 5 and 6. Secondly, we showed that the ~30 nt RNA at upstream end of exon 4 and the ~33 nt RNA at downstream end of exon 7 also modulate splicing of exon 5 and 6. Thirdly, our results indicate that the RNA sequences of the ends in exon 4 and 7 are required for the regulatory functions of the RNA from restriction enzyme cleavage sites. Our results provide a new insight for regulation of alternative splicing of Ron proto-oncogene.

Keywords: Ron, pre-mRNA splicing, Regulatory-RNA sequence, Ron, exon 5, exon 6


Citation styles

APA
Moon, H., Zheng, X., Loh, T.J., Jang, H.N., Liu, Y., Jung, D.W., Williams, D.R., Shen, H. (2015). Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene. Journal of Cancer, 6(12), 1346-1351. https://doi.org/10.7150/jca.13361.

ACS
Moon, H.; Zheng, X.; Loh, T.J.; Jang, H.N.; Liu, Y.; Jung, D.W.; Williams, D.R.; Shen, H. Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene. J. Cancer 2015, 6 (12), 1346-1351. DOI: 10.7150/jca.13361.

NLM
Moon H, Zheng X, Loh TJ, Jang HN, Liu Y, Jung DW, Williams DR, Shen H. Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene. J Cancer 2015; 6(12):1346-1351. doi:10.7150/jca.13361. https://www.jcancer.org/v06p1346.htm

CSE
Moon H, Zheng X, Loh TJ, Jang HN, Liu Y, Jung DW, Williams DR, Shen H. 2015. Identification of Regulatory-RNAs for Alternative Splicing of Ron Proto-Oncogene. J Cancer. 6(12):1346-1351.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.