1. Department of General Surgery, Xiangya Hospital, Central South University, Changsha, China.
2. Key Laboratory of Nanobiological Technology of National Health and Family Planning Commission, Xiangya Hospital , Central South University, Changsha, China.
3. Institute of Reproductive and Stem Cell Engineering, School of Basic Medicine, Central South University, Changsha, China.
4. Xiangya Stomatological Hospital & School of Stomatology, Central South University, Changsha, China.
Through bioinformatics analysis, a novel lncRNA, LINC00460, was implicated in the development of multiple cancers. However, the precise expression pattern, clinical significance and biological function of LINC00460 in colorectal cancer (CRC) remain unknown. Network databases were used to investigate the correlation between LINC00460 and CRC. In situ hybridization was performed to verify the precise expression pattern and clinical significance of LINC00460 in a CRC tissue microarray, which included 92 pairs of CRC and adjacent normal tissues. The effect of LINC00460 on proliferation was evaluated by MTT, colony formation assays and flow cytometry employing SW620 and HCT116 cell lines. Cell migration and matrigel invasion assays were performed to investigate whether LINC00460 is involved in the metastasis of CRC. The expression of LINC00460 was significantly upregulated in CRC tissues and cells, associated with early stage CRC and low disease-free survival. The downregulated of LINC00460 expression increased cell proliferation by regulating the cell cycles of SW620 and HCT116 cells. LINC00460 knockdown did not affect cell migration or invasion in vitro. These findings suggest that LINC00460 may be an interesting target for the development of CRC.
Keywords: long non-coding RNA, LINC00460, colorectal cancer, proliferation