J Cancer 2020; 11(6):1625-1633. doi:10.7150/jca.39359

Research Paper

LZAP promotes the proliferation and invasiveness of cervical carcinoma cells by targeting AKT and EMT

Yi-Fang Dai1,2, Na Lin1,2, De-Qin He1,2, Mu Xu3, Li-Ying Zhong3, Shu-Qiong He1,2, Dan-Hua Guo1,2, Ying Li1,2, Hai-Long Huang1,2, Xiang-Qing Zheng3✉, Liang-Pu Xu1,2✉

1. Center of Prenatal Diagnosis, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou 350001, China
2. Fujian Provincial Key Laboratory for Prenatal diagnosis and Birth Defect, Fuzhou 350001, China
3. Department of Gynecology, Fujian Provincial Maternity and Children's Hospital, Fuzhou 350001, China

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Citation:
Dai YF, Lin N, He DQ, Xu M, Zhong LY, He SQ, Guo DH, Li Y, Huang HL, Zheng XQ, Xu LP. LZAP promotes the proliferation and invasiveness of cervical carcinoma cells by targeting AKT and EMT. J Cancer 2020; 11(6):1625-1633. doi:10.7150/jca.39359. Available from https://www.jcancer.org/v11p1625.htm

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Abstract

Objective: To explore the relationship and mechanism of LZAP in the occurrence and development of cervical cancer and to provide a new target and intervention method for the treatment of cervical cancer.

Methods: Data mining and analysis of LZAP expression levels were performed using several online databases, including The Cancer Genome Atlas (TCGA). A cervical cancer cell line that stably overexpresses LZAP was established, and the effect of LZAP overexpression on cell proliferation, invasion, migration and tumor formation in vivo as well as its mechanism were explored.

Results: Our study shows that the expression of LZAP is upregulated in cervical cancer. The overexpression of LZAP can significantly promote the proliferation, colony formation, and invasion and migration abilities of cervical cancer cells. The tumorigenesis test in nude mice showed that overexpression of LZAP could promote the tumorigenicity of cervical cancer cells in vivo. LZAP could also promote the phosphorylation of AKT at position 473 and the epithelial-mesenchymal transition (EMT).

Conclusion: The expression of LAZP is increased in cervical cancer, which can enhance the invasion, metastasis, and EMT in cervical cancer cells by promoting AKT phosphorylation.

Keywords: LZAP, EMT, Cervical Carcinoma, Metastasis