J Cancer 2020; 11(7):1907-1912. doi:10.7150/jca.39722 This issue

Research Paper

Circular RNA CircHIPK3 Promotes Gemcitabine Sensitivity in Bladder Cancer

Fang Xie1, Ning Zhao2, Hui Zhang3, Dalong Xie4✉

1. Medical Basic Experimental Teaching Center, China Medical University, Shenyang, 110122, China
2. Surgery Laboratory, Affiliated First Hospital, China Medical University, Shenyang, 110001, China
3. Department of Urinary surgery, Shengjing Hospital, China Medical University, Shenyang, 110004, China
4. Department of Anatomy, College of Basic Medicine, China Medical University, Shenyang, 110122, China

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Citation:
Xie F, Zhao N, Zhang H, Xie D. Circular RNA CircHIPK3 Promotes Gemcitabine Sensitivity in Bladder Cancer. J Cancer 2020; 11(7):1907-1912. doi:10.7150/jca.39722. Available from https://www.jcancer.org/v11p1907.htm

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Abstract

Purpose: Recent studies showed circular RNA (circRNA) played important regulatory roles in tumors, including genesis of chemotherapy resistance. In this study, the role of circHIPK3 on chemotherapy resistance of bladder cancer (BC) will be clarified.

Methods: Real-time quantitative PCR was applied to examine the circHIPK3 expression. The gemcitabine sensitivity and cell proliferation viability were analyzed by Cell Counting Kit-8 assay. Double-stained flow cytometry was used to detect the cell apoptosis.

Results: In BC tissues and cell lines, the circHIPK3 expression was down-regulated. Its expression had a negative correlation with pathological grade, lymph node metastasis and gemcitabine insensitivity of BC patients. CircHIPK3 was a independent prognostic biomarker for BC patients. The expression of circHIPK3 in T24/gem and J82/gem cell lines (resistant to gemcitabine) was down-regulated significantly. The over-expression of circHIPK3 decreased IC50 of gemcitabine and promoted gemcitabine's cytotoxicity in T24/gem and J82/gem cells.

Conclusions: The circHIPK3 is low-expressed in BC and is an independent prognostic biomarker for BC patients. The low-expression of circHIPK3 is associated with the insensitivity to gemcitabine of BC patients, over-expression of circHIPK3 promotes gemcitabine sensitivity in BC.

Keywords: bladder cancer, circular RNA, circHIPK3, chemotherapy, gemcitabine